大绿菇菌丝体富锌能力的研究  

Studies on Zn accumulation of russula virescens

作　　者：董学卫[1] 朱启忠[1] 郭锦 郭晋东 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《食品与机械》2007年第5期17-19,22,共4页Food and Machinery

摘　　要：对大绿菇菌丝体进行液体富锌培养。首先对大绿菇的耐锌和富锌特性进行研究,结果表明:大绿菇具有很强的耐锌能力和富锌能力,其菌丝体在锌浓度为50～2000mg/L的固体培养基上均能够生长;菌丝体对锌的最适富集质量浓度为200mg/L,超过300mg/L锌质量浓度对菌丝体的生长有较大影响。本试验还对富锌培养条件进行优化,在培养温度26℃,起始pH5～6,振荡速度100r/min,250mL三角瓶装液75mL,接种量15%(V∶V),培养基中锌添加量为200mg/L时,大绿菇菌丝的生物转化量及菌丝体富锌率最高,此时菌丝体生物量达到8.79g/L,菌丝体含锌量为2.530mg/g,富锌率为10.51%。对锌的有机化研究结果表明:大绿菇能将锌转化形成体内共价结合的有机锌,而不是简单的物理吸附,且其有机化程度在81.6%左右。Russula virescens was cultured in liquid medium containing Zn ions.The Zn-resisting and Zn-accumulating characters of submerged culture Russula virescens were studied in this paper.The results showed that the capacity of both Zn-resisting and Zn-accumulating of Russula virescens was high.The strain can grow in the solid medium with Zinc concentrition from 50 to 2 000 mg/L.However,the Zinc content above 200 mg/L hampered its growth.When zinc added to the liquid medium up to 200 mg/L,the hyphae have the most ...

关 键 词：大绿菇 锌 富集 

分 类 号：S646[农业科学—蔬菜学]