HBV C区基因扩增与PCR条件的优化  

Genes of HBV C Region Amplifying and Optimization of PCR Conditions

作　　者：马兆武[1] 袁媛[2] 陈强[2] 陈思礼[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]华中师范大学生命科学学院,武汉430079

出　　处：《中南民族大学学报（自然科学版）》2007年第1期30-32,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家民委自然科学基金资助项目(MZZ04002);中南民族大学自然科学基金资助项目(YZZ04006)

摘　　要：目的:为提高PCR检测乙型肝炎病毒(HBV)的特异性和灵敏度,降低成本,对PCR条件进行优化.方法:将HBV特异性基因片段转化到大肠杆菌DH5α中,提取质粒,利用PCR扩增HBV C区基因,对PCR条件中的退火温度、Mg2+浓度进行优化,并比较3种不同Taq DNA聚合酶的灵敏度.结果:HBV C区基因扩增的最佳退火温度为58℃,最佳Mg2+浓度为1.5mmol/L,Biostar Taq DNA聚合酶扩增到10-6.讨论:对HBV C区基因进行了转化和PCR实验条件的优化,为扩大PCR在乙型肝炎病毒(HBV)检测领域中的应用提供实验依据.To increase the speciality and sensitivity of PCR for the genes of HBV C region and to decrease the cost of PCR detecting HBV and to optimize the PCR conditions.Methods: HBV genes were transformed into E.coli,DH5α.Plasmid was extracted from those E.coli. And the genes of HBV C region were amplified.Then optimizing the temperature of annealing and Mg2+ density,and comparing the sensitivities of three kinds of different Taq DNA polymerase.The results showed that the optimal annealing temperature was 58℃,the o...

关 键 词：乙型肝炎病毒 聚合酶链反应 条件优化 

分 类 号：N[自然科学总论]