影响羊早期胚胎发育阻滞相关基因的筛选  

Screening of goat embryo development block related genes

作　　者：李拥军[1] 敖红[2] 孙桂金[2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院畜牧研究所,北京100094

出　　处：《扬州大学学报（农业与生命科学版）》2007年第4期25-28,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30270162);北京市自然科学基金资助项目(6032019)

摘　　要：采用单胚构建的mRNA差异显示技术,探讨经体内发育和体外培养获得的山羊8~16细胞期胚胎基因的表达情况,并从在体内发育胚胎特异表达的片段中选择1个片段进行分析。结果表明:该片段与牛肥胖基因具有86%的同源性。该基因对卵母细胞的成熟以及早期胚胎的分裂和分化具有明显的作用,可调节母体的妊娠生理,影响生殖激素的分泌,从而对克服早期胚胎发育阻滞具有重要的作用。In order to study the mechanism about the development block of goat preimplatantion embryos,the goat 8-16 cells stage embryos obtained from in vivo and in vitro were studied using the way of single preimplantation embryo mRNA differential display.Some specific fragments expressed in in vivo embryos were found and one of them was selected for further analysis.The result suggested that this fragment displayed high homology(86%) to cattle leptin(Obese) gene.Leptin had the effects on oocytes maturation,embryoni...

关 键 词：山羊 胚胎 单胚差异显示技术 发育阻滞 基因 

分 类 号：S826[农业科学—畜牧学]