蜡蚧轮枝菌昆明菌株可湿性粉剂的发酵培养条件及其毒力测定  

Formulation Technology and Application of Verticillium lecanii Wettable Powder

作　　者：张亚平[1] 徐启高[1] 杨美林[1] 周天雄[1] 

机构地区：[1]云南农业大学植物保护学院,昆明650201

出　　处：《中国生物防治》2007年第S1期28-31,共4页Chinese Journal of Biological Control

基　　金：省教育厅科学研究基金项目(0112316);教育部重点基金项目资助(02143)

摘　　要：采用价格低廉的农副产品结合液固两相培养的方法进行发酵培养,干燥后的固体培养基经粉碎后人工过300目筛,取菌粉(55%),加过300目筛的填料硅藻土(42%)、润湿剂十二烷基苯磺酸钠(3%)及稳定剂混匀制得55%蜡蚧轮枝菌可湿性粉剂。该制剂有效孢子浓度3.0×109个/g;细度:97%以上过300目筛;润湿时间:2′57″;悬浮率:75.6%;含水量<3.0%;pH值6~7。该制剂对小菜蛾的LC50到8d时为1.51×104孢子/ml;在田间试验中,施用3次该可湿性粉剂2.1×107孢子/ml对萝卜蚜的15~25d防治效果为64.9%~70.7%。Using farm and sideline products as culture medium to cultivate Verticillium lecanii,then shatter it to obtain powder.55% powder,42% diatomite,3% SDBS and stabilizing agent constitute 55% V.lecanii wettable powder.This formulation s effective spores concentration is 3.0×109 spore/g;diameter of 97% of formulation is 0.05 mm;wettable time is 2 min and 57 seconds;suspension percentage is 75.6%;water capacity <3.0% and it s pH is 6-7.LC50 of the wettable powder to Plutella xylostella can reach to 1.51×104 sp...

关 键 词：蜡蚧轮枝菌 可湿性粉剂 小菜蛾 毒力测定 萝卜蚜 田间药效试验 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]