薄层层析-光度法联用测定甲基对硫膦中o,o′-二甲基-对硝基苯基硫代膦酸酯  

Determination of o,o′-Dimethyl-p-Nitrophenyl Thiophosphonate in Methyl Parathion by Photometry Hyphenated with Thin Layer Chromatography

作　　者：张锂[1] 韩国才[1] 

机构地区：[1]兰州工业高等专科学校基础学科部

出　　处：《理化检验（化学分册）》2007年第12期1059-1061,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：应用薄层色谱法(TLC)使对硫膦中的主成分,o,o′-二甲基-对硝基苯基硫代膦酸酯(DMNPTP),与以杂质共存的二硝基与邻硝基衍生物分离。TLC法中用硅胶(G254)作吸附剂,用石油醚与乙酸乙酯(8+2)的混合物作为展开溶剂,经TCL分离后,用刀片将样品(含DMNPTP)的色谱带括下,经蒸发除去有机溶剂,用硝酸-高氯酸加热消化并蒸发至干后,用硝酸溶解残渣。此时,溶液中原来以DMNPTP存在的化合物已转化为正磷酸盐,随即用磷钒钼杂多酸光度法测定其磷含量,测定波长为其吸收峰450 nm,由测定值换算为DMNPTP的含量。Thin layer chromatography(TLC) was used to separate the main component,o,o′-dimethyl-p-nitrophenyl thiophosphonate(DMNPTP),from the dinitro and o-nitro derivatives which are present as impurities in methyl parathion.Silica gel(G254) was used as the adsorbent and a mixture of petroleum ether and ethylacetate(8+2) was used as the spreading solvent.After separation by TLC,the chromatographic band of the sample which contains the DMNPTP,was taken down from the TLC plate with a razor blade.After removing the org...

关 键 词：光度法 薄层色谱法 O o′-二甲基-对硝基苯基硫代膦酸酯 甲基对硫膦 

分 类 号：O657.3[理学—分析化学]