转基因抗穗发芽小麦的获得  

The study on transgenic wheat with the characteristic of pre-harvest sprouting resistance

作　　者：尹钧[1] 任江萍[1] 李志岗[1] 李磊[1] 周苏玫[1] 卫丽[1] 宋丽[1] 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,河南郑州450002

出　　处：《湖南农业大学学报（自然科学版）》2007年第S1期67-71,85,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金(30370877);河南省杰出人才创新基金(121000700)

摘　　要：以易穗发芽小麦幼胚为受体,用基因枪法对TrxS反义基因(目的基因)和Bar基因(标记基因)进行了共转化;通过对轰击后的小麦幼胚进行愈伤组织诱导、胚性愈伤组织分化和生根培养,获得再生植株;经分子检测证实在3个受体材料上获得转基因再生植株和可遗传的转基因后代.通过培养皿籽粒发芽试验、离体整穗保湿发芽试验和温室模拟降雨穗发芽试验,均证明转基因后代材料具有延缓籽粒发芽和抗穗发芽的特性.Wheat(Triticum aestivum L) pre-harvest sprouting,a world problem,effects the grain quality,yield and economic value.In the study,the easily pre-harvest sprouting,white seed varieties were selected as receptor materials.Antisense TrxS gene and Bar gene were co-transformed to immature embryos of wheat via microprojectile-bombardment.The bombarded immature embryos were cultured for inducing callus,callus differentiation and plant regeneration and the regenerated plants were obtained.The molecular assay of the ...

关 键 词：小麦穗发芽 基因枪转化 TrxS反义基因 转基因小麦 

分 类 号：S512.1[农业科学—作物学]