玉米ZmPTF1基因克隆和过表达分析  

Cloning of ZmPTF1 from zea mays and its overexpression analysis

作　　者：李朝霞[1] 高强[1] 刘雅正[1] 何春梅[1] 张举仁[1] 

机构地区：[1]山东大学生命科学学院,山东济南250100

出　　处：《湖南农业大学学报（自然科学版）》2007年第S1期92-96,共5页Journal of Hunan Agricultural University(Natural Sciences)

摘　　要：PTF是一类具有bHLH结构域的转录因子,在低磷胁迫应答中调节一系列下游磷胁迫基因的表达.根据拟南芥和水稻中的序列信息,通过RACE的方法获得ZmPTF1的全长cDNA序列.序列分析表明该1709bp的cDNA片段含有1446bp的完整开放读码框,编码481个氨基酸的多肽链,含有一个bHLH结构域,与已报道的OsPTF1在氨基酸水平上有65%的相似性.构建了pCAMBIA1300-Pubi-ZmPTF1-Tnos-als质粒转化玉米,得到过表达的转基因后代.对低磷营养液浇灌下的T2代植株分析表明:过表达ZmPTF1促进了植株根系的发育,提高了植株对低磷环境的耐受性.PTF is a transcription factor with a basic helix-loop-helix domain for tolerance to inorganic phosphate(Pi) starvation.Based on the sequences of Arabidopsis thaliana and Oryza sativa,a pair of primers(P1 and P2) for RT-PCR was designed and synthesized according to these conserved sequences.The full-length cDNA was obtained from ZmcDNA by 5 RACE.Sequence analysis showed that it encoded the bHLH conserve domain.Sequence analysis showed that this cDNA fragment was 1 709 bp long with an open reading frame of 1 ...

关 键 词：ZmPTF1 玉米 转基因 耐低磷 

分 类 号：S513[农业科学—作物学]