籼稻成熟胚的组织培养特性研究  

Studies on the tissue culture characteristic of mature embryos of indica rice

作　　者：徐庆国[1] 殷绪明[1] 李海林[1] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2007年第S1期5-10,共6页Journal of Hunan Agricultural University(Natural Sciences)

摘　　要：籼稻成熟胚培养由于取材容易,因此在转基因研究中具有广泛的应用前景.但籼稻成熟胚愈伤组织诱导率不高,继代易褐化,分化率不高,严重阻碍着基因工程技术在水稻育种中的应用.本研究选用了27不同类型的籼稻品种和常规粳稻品种作为实验材料,在同一培养方式下,分析了不同籼稻品种成熟胚愈伤组织诱导率和分化率之间的差异及籼稻品种与粳稻品种成熟胚愈伤组织诱导率与分化率之间的差异,为目前应用细胞工程和基因工程技术来改良籼稻品种提供理论依据.获得了如下主要研究结果:水稻基因型是影响籼稻成熟胚组织培养力高低的关键因素;籼型杂交水稻不育系和保持系比常规籼稻品种、杂交水稻F1种子和恢复系品种成熟胚的培养力低;杂交水稻F1种子受亲本恢复系基因型的影响较大.Mature embryo of Indica Rice has vast application prospects in transgenic studying,because it can be get freely.but the induction rate and the differentiation frequency of callus from mature embryo of Indica Rice is very low,the frequency of callus browning is high in subculture period.All this is a barrier to the application of genetic engineer to rice breeding.27 different Indica Rice varieties and Japonica rice varieties were used as material to analyze the difference of induction and differentiation of ...

关 键 词：籼稻 成熟胚 组织培养 基因型 

分 类 号：S511.21[农业科学—作物学]