线性扫描伏安法测定人血清白蛋白  

Linear Sweeping Voltammetric Determination of Human Serum Albumin and Its Analytical Application

作　　者：王学亮[1] 焦奎[2] 

机构地区：[1]菏泽学院化学与化工系,菏泽274015 [2]青岛科技大学化学与分子工程学院,青岛266045

出　　处：《理化检验（化学分册）》2007年第11期940-942,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(20075013)资助项目

摘　　要：建立了一种测定人血清白蛋白的新方法。应用线性扫描伏安法研究了灿烂甲酚蓝(BCB)与人血清白蛋白(HSA)的相互作用的条件,当BCB与HSA作用后,其峰电流下降且峰电位负移,峰电流的下降值的二阶导数(ΔI″p)同HSA的质量浓度在2.0~60.0 mg.L-1范围内呈线性关系,相关系数为0.9970。方法的检出限为2.0 mg.L-1,用于健康人血清样品的测定,回收率在95.6%~107.5%之间。Linear sweeping voltammetry was applied to the determination of human serum albumin(HSA) bese on its interaction with brillianl cresol blue(BCB).Conditions for the reaction were studied and optimized. In a B-R buffer medium of pH 6.5,reductive peak of BCB was observed at-0.1 V(vs.SCE),and in the presence of HSA,due to their interaction,the reductive peak was shifted negatively with decrease in peak current.Linear relationship between 2nd derivative of the magnitude of decrease in peak current(ΔI″p) and the ...

关 键 词：线性扫描伏安法 灿烂甲酚蓝 人血清白蛋白 临床检验 

分 类 号：R446.1[医药卫生—诊断学]