弱光逆境诱导葡萄叶片内肽酶活性变化的研究  

Effects of Weak Light Stress on Endopeptidases Activity of Grape Leaves

作　　者：曹慧[1] 郭伟[1] 王效忠[1] 陈爱军[1] 

机构地区：[1]潍坊学院,山东潍坊261061

出　　处：《潍坊学院学报》2007年第4期78-80,共3页Journal of Weifang University

基　　金：潍坊市科技发展计划项目资助

摘　　要：葡萄叶片在弱光胁迫下内肽酶活力的变化可分为低值缓升期和速升期,对应于叶绿素含量缓降期和速降期,用激活剂和抑制剂处理,结果表明弱光胁迫引起叶片老化过程中存在3种以上的内肽酶(巯基蛋白酶、丝氨酸蛋白酶和金属蛋白酶),而在蛋白质降解中起主要作用的是巯基蛋白酶。The changes of endopeptidases activities in grape leaves under poor light stress included two phases,low steady phase and rapid increase phase,which relatively consisted with the changes of chlorophyll contens.Identified by inhibitors(IA,PMSF,EDTA),activator(DTT)and different substrates,there existed more than three subclasses of endopepidases during leaf aging process,namely thiol-proteinases,serine proteinases,and metallo-proteinases,and among of them,thiol-proteinases was the most active one.

关 键 词：弱光逆境 葡萄 内肽酶 

分 类 号：Q946.5[生物学—植物学]