915MHz电磁波对小鼠脑内神经递质含量的影响  

Effects of 915 MHz Electromagnetic Fields on the Concentration of Neurotransmitters in the Brain Tissue of the Mice

作　　者：王焱[1] 曹兆进[1] 王强[1] 张淑珍[1] 

机构地区：[1]中国疾病预防控制中心公共卫生管理处,北京100050

出　　处：《环境与健康杂志》2007年第10期768-770,共3页Journal of Environment and Health

摘　　要：目的研究915MHz电磁波对小鼠脑组织内神经递质含量的影响,探索微波辐射对中枢神经系统影响的机制。方法将1月龄清洁级健康雄性昆明小鼠随机分为高剂量组(5mW/cm2)、低剂量组(2mW/cm2)、对照组(除不进行电磁波辐射外,其余条件均与剂量组相同),每天从9:00开始,依次轮流暴露于915MHz电磁场中,每组暴露2h,连续暴露30d。采用高效液相色谱-电化学法测定脑皮层、海马中的单胺类神经递质[去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)]及其代谢产物[多巴胺的代谢产物:3,4-二羟基苯乙酸(DOPAC),5-羟色胺的代谢产物:5-羟基吲哚乙酸(5-HIAA)]含量,采用碱性羟胺比色法测定剩余脑组织中乙酰胆碱(Ach)含量。结果不同组别小鼠脑皮层、海马内NE、DA(海马内DA低于仪器检出限,不作比较)、5-HT、DOPAC、5-HIAA含量间比较,经方差分析,差异无统计学意义(P>0.05)。不同组别小鼠脑组织内乙酰胆碱(Ach)含量间比较,经方差分析,差异无统计学意义(P>0.05)。结论经过915MHz的电磁辐射,未发现小鼠大脑皮层、海马内的单胺类神经递质及其代谢产物含量以及脑组织内Ach含量发生改变。Objective To study the effect of 915 MHz electromagnetic fields on the concentration of neurotransmitters in the brain tissue of the mice,to investigate the mechanism of effects of microwave on central nervous system(CNS). Methods 30 one-month-old SPF-level Kunming male mice were divided randomly into high-dose group, low-dose group and control group. From 9 o'clock everyday in the morning,they were exposed to 915 MHz electromagnetic fields for 2 hours in turn during the continuous 30 days,the power density of which were 5 mW/cm2,2 mW/cm2 and 0 mW/cm2. The concentration of monoamine neurotransmitters(NE, DA, 5-HT) and metabolite (DOPAC, 5-HIAA) in the cerebral cortex and hippocampus were detected by high performance liquid chromatography with electrochemical detector(HPLC-ECD).The concentration of Ach in the remnant brain tissue was detected by spectrophotometer. Results The concentration of monoamine neurotransmitters(NE,DA,5-HT),DOPAC (metabolite of DA) and 5-HIAA(metabolite of 5-HT) in the cerebral cortex were not significantly different among the three groups using the analysis of variance (P>0.05). The concentration of monoamine neurotransmitters(NE,5-HT),DOPAC and 5-HIAA in the hippocampus were not significantly different(P>0.05).The concentration of Ach in the remnant brain tissue of the three groups was not significantly different(P>0.05). Conclusion After exposing to the 915 MHz electromagnetic fields, the changes of the concentration of monoamine neurotransmitters in the cerebral cortex and hippocampus, and the changes of the concentration of Ach in the remnant brain tissue could not be found.

关 键 词：辐射 神经递质 皮层 海马 

分 类 号：R994.6[医药卫生—毒理学]