二羟环氧苯并芘对人支气管上皮细胞N-Ras基因表达的影响  

Effect of BPDE on Expression of N-Ras Gene in Human Bronchial Epithelial Cells

作　　者：周兰兰[1] 蒋义国[1] 沈月兰[1] 傅娟[1] 曹虹[2] 

机构地区：[1]广州医学院化学致癌研究所与预防医学系,广东广州510182 [2]江西省人民医院普外科,江西南昌330006

出　　处：《环境与健康杂志》2007年第12期948-950,封3,共4页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30571546;30771780);教育部留学回国人员科研启动基金资助项目(2007-24);广东省高校自然科学重点项目(06Z021);广东省自然科学基金资助项目(07117550)

摘　　要：目的探讨环境致癌物苯并(a)芘代谢物二羟环氧苯并芘(BPDE)对人支气管上皮细胞N-Ras基因表达的影响。方法利用RT-PCR和Western blot方法,分别检测N-Ras基因在经BPDE恶性转化细胞中mRNA和蛋白表达水平的变化。利用免疫细胞化学方法检测N-Ras基因在经BPDE恶性转化细胞中定位和蛋白表达量的变化。结果RT-PCR和Western blot实验表明,BPDE恶性转化细胞N-Ras基因的mRNA和蛋白水平分别是正常细胞的3.616和1.600倍。免疫细胞化学实验显示,在BPDE恶性转化细胞和正常细胞中,N-Ras基因在细胞膜和细胞浆中均有表达,且两者相比,前者中的N-Ras蛋白表达明显强于后者。结论N-Ras基因可能在BPDE的致癌机制中起着重要作用。Objective To explore the effect of BPDE on the expression of N-Ras in the human bronchial epithelial cell line. Methods The levels of mRNA and protein expression in BPDE transformed 16HBE cells(16HBE-T) and untransformed control 16HBE cells(16HBE-N) were examined by using RT-PCR and Western blot. Locations and expression levels of protein in both kinds of cells were analyzed by immunocytochemical method. Results Compared with 16HBE-N, the levels of mRNA and protein of N-Ras significantly increased to 3.616 and 1.600 times in 16HBE-T. Immunocytochemical method showed N-Ras protein in 16HBE-T and 16HBE-N expressed in the cytomembrane and cytoplasm, but the expression level of protein in 16HBE-T was significantly higher than that in 16HBE-N. Conclusion The up-regulated expression of N-Ras oncogene may play an important role in the malignant transformation of 16HBE induced by BPDE

关 键 词：空气污染 N-RAS基因 二羟环氧苯并芘 人支气管上皮细胞 

分 类 号：R994.6[医药卫生—毒理学]