根瘤内生细菌对大豆胞囊线虫及根腐病菌的影响  

EFFECT OF ENDOPHYTIC BACTERIA FROM SOYBEAN ROOT NODULES ON SOYBEAN CYST NEMATODE AND PATHOGENS OF SOYBEAN ROOT ROT

作　　者：王媛媛[1] 段玉玺[1] 陈立杰[1] 

机构地区：[1]沈阳农业大学植物保护学院线虫学研究室,沈阳110161

出　　处：《大豆科学》2007年第2期213-217,共5页Soybean Science

基　　金：国家自然科学基金(20372046;30300231);科技部成果转化基金(05EFN212100059);农业部成果转化基金

摘　　要：为了明确大豆根瘤内生芽孢杆菌Snb2对大豆胞囊线虫的毒性和大豆根腐病菌的抑制作用,用菌悬液处理和对峙培养法分别测定了Snb2对两种病原微生物的作用效果。结果表明:Snb2的菌悬液能够明显抑制大豆胞囊线虫胞囊的孵化,相对抑制率达到94.9%;菌悬液处理J2 96 h时死亡率达到66.7%;Snb2菌株对4种大豆根系病原真菌表现不同程度的拮抗作用,对尖孢镰刀菌和茄腐镰刀菌的拮抗作用最明显,抑菌圈达到10 mm左右,抑制作用可持续10 d;经细菌悬浮液浸种测定,处理后的大豆子叶节到根尖的距离为9.1±4.54 cm,较对照增加了15.19%%,对幼苗生长有明显的促进作用;通过温室盆栽防效试验,进一步表明Snb2菌悬液进行种子浸种对大豆胞囊线虫病有明显的抑制作用,防治效果达到62.5%。In order to study the effect of endophtic bacteria Snb2,Bacillus sp.,from soybean root on Heterodera glycines and soybean root rot pathogens,treatment J2 and cyst with bacterial suspension and Dual-culture tests to soybean root rot pathogens were developed.The result showed that the bacterial suspension had effect on the hatching of cyst and the rate of inhibition was 94.9%;after 96 h,the mortality of J2 was 66.7% treated with bacterial suspension;strain Snb2 had strong anti-fungal activity to 4 soybean root rot pathogens,especially to Fusarium oxysporum,Fusarium solani,the inhibition zone was 10 mm and lasted almost ten days;after treated with bacterial,it apparently promoted the growth of soybean seedling,the length between cotyledon and root tip was 9.1±4.54 cm,15.19% higher than that of CK;In potted trial,bacterial suspension showed obvious efficency against soybean cyst nematode and control efficercy reached 62.5%.

关 键 词：根瘤 内生芽孢杆菌 促生 大豆胞囊线虫 大豆根腐病菌 拮抗 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]