文昌鸡GnRHR基因在不同组织中差异表达的研究  

Differential Expression of GnRHR Gene among Different Tissues in Wenchang Chicken

作　　者：严美姣[1] 陈宽维[2] 吴旭[1] 钱凯[1] 朱文奇[1] 俞亚波[1] 张晶鑫[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《中国家禽》2007年第23期18-20,共3页China Poultry

摘　　要：采用半定量RT-PCR方法检测文昌鸡处于性腺轴(下丘脑-垂体-性腺)的不同组织中GnRHR基因的表达水平,结果表明:GnRHR基因mRNA表达于鸡的3种组织——下丘脑、垂体和卵巢;GnRHR mRNA在3种组织中的表达量不同:下丘脑和垂体中表达量较高,卵巢中表达量较弱。Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify GnRHR gene expression level in different tissue of gonadal axis(hypothalamus, pituitary,gonad) in Wenchang chicken. The results showed that GnRHR mRNA gene had been found to express in hypothalamus,pituitary and ovary;the expression quantity was different among three tissues,the value was lower in ovary than in other two tissues. This work laid the foundation for further research into the control of reproduction in chicken, such as the functional significance of multiple GnRHR in vertebrates,and the molecular mechanisms controlling tissue specific GnRHR expression.

关 键 词：GnRHR基因 差异表达 文昌鸡 

分 类 号：S83[农业科学—畜牧学]