猪细小病毒SX株的分离鉴定  

Isolation and Identification of Porcine Parvovirus(PPV) Strain SX

作　　者：刘业兵[1] 张万林[2] 范运峰[1] 郑杰[1] 宁宜宝[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]乾元浩生物股份有限公司北京厂,北京100081

出　　处：《中国兽药杂志》2007年第12期13-15,共3页Chinese Journal of Veterinary Drug

摘　　要：从发生蓝耳病的猪场采集流产、死胎病料中分离出了一株病毒,适用传代细胞后,该病毒能够在ST细胞上生长并引起明显的呈拉网状和细胞脱落的细胞病变(CPE),能够凝集0.5%的豚鼠红细胞,经猪细小病毒阳性血清中和后不能引起细胞病变(CPE),也失去了血凝特性;用特异荧光抗体染色可见细胞核内出现特异性荧光。采用特异性PPV NS1基因的引物进行扩增,得到了大小约为2.18 kb的片段,测序并与猪细小病毒相应序列进行比较,同源性为99%以上,鉴定该病毒为猪细小病毒,命名为PPV-SX。说明目前有部分猪繁殖障碍是由猪细小病毒和PRRS共同感染而致。One porcine parvovirus(ppv) strain was isolated successfully from the embryonic resorption,fetal mummification,abortion and stillbirth of breakout porcine reproductive and respiratory syndrome pig farm.This strain could multiply in ST cell and produce prominent CPE: the infected cells appeared gathering and amalgamation.This strain also could agglutinate the RBC of guinea pig and could be inhibited by the positive ppv serum.Fluorescence assay was used to detect the antigens of porcine parvovirus(PPV) with bright green fluorescence in the infected cells.The PCR assay was established to amplify NS1gene of PPV.A 2.18 kb fragment was achieved and sequenced.It was identified as porcine parvovirus by blast on the NCBI and named as PPV-SX.

关 键 词：猪细小病毒 PPV-SX 分离 鉴定 

分 类 号：S852.659.2[农业科学—基础兽医学]