高效液相色谱法测定复方呋已栓三主药的含量  

HPLC Determination of Nitrofural and Diethylstilbestrol and Tinidazole in Compound Fuyi Suppsitory

作　　者：尹艳莲[1] 李爱英[1] 

机构地区：[1]河北工程学院附属医院,河北邯郸056029

出　　处：《中国现代应用药学》2007年第z1期636-637,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立高效液相色谱法测定复方呋已栓中替硝唑、已烯雌酚和呋喃西林含量的方法。方法用Hypersil C18(250mm×4.6mm,5μm)色谱柱;以甲醇-水-乙醚(30:70:1)为流动相;流速为1.0mL.min-1;柱温为20℃;检测波长为263nm。结果替硝唑、已烯雌酚和呋喃西林的保留时间分别为4.23,5.42,6.35min,理论塔板数分别为5320,2450和3560;HPLC法测定的线性范围分别在192.1～448.1μg.mL-1(r氯=0.9999),5.8～17.2μg.mL-1(r乙=0.9995)和9.7～22.6μg.mL-1(r呋=0.9998);平均回收率分别为100.4%,99.99%和101.5%,RSD分别为1.2%(n=5),0.576%(n=5)和1.8%(n=5);该法的精密度和稳定性良好。结论本法用于复方呋已栓中替硝唑、呋喃西林和已烯雌酚的含量测定简便、快速、准确、可靠。OBJECTIVE To establish a HPLC method for content detenmination of nitrofural and diethylstilbestrol and tinidazole in Compound Fuyi Suppsitory.METHODS The Hypersil C18colunm was used,the mobile phase was methanol-water-ether(30:70:1);The flow rate:1.0 mL·min-1;The column temperature:20℃;the defecting wavelength:263 nm.RESULTS The retention times for nitrofural and diethylstilbestrol and tinidazole were 4.23 min and 5.42 min and 6.35 min with theoretical plates were 5320 and 2450 and 3560;respectively.There were good linear relationship with in the range of 192.1~448.1 μg·mL-1(r氯=0.999 9),5.8~17.2 μg·mL-1(r乙 =0.999 5),5.8~17.2 μg·mL-1(r乙 =0.999 5)和9.7~22.6 μg·mL-1(r曲=0.999 9).The precision and and repeatability of the method was good,RSD were 1.2%(n=5),0.576%(n=5) and 1.8%(n=5).CONCLUSION The method is convenient,rapid,accurate and reliable may by adopted for assay of nitrofural and diethylstilbestrol and tinidazole in Compound Fuyi Suppsitory.

关 键 词：复方呋已栓 替硝唑 已烯 雌酚 呋喃西林 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]