地非三唑抑制血管新生作用的研究  

Antiangiogenesis Effect of DL111-IT in Vivo and in Vitro

作　　者：李晴宇[1] 王刚[1] 

机构地区：[1]浙江省杭州市第一人民医院药剂科,杭州310006

出　　处：《中国现代应用药学》2007年第z2期688-691,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 从细胞、器官、整体三个水平观察地非三唑抑制血管的新生作用.方法 细胞水平用MTT法测定地非三唑对人脐静脉内皮细胞的增殖抑制作用;器官水平上利用鸡胚绒毛尿囊膜(CAM)血管新生模型,观察地非三唑抑制CAM血管新生的情况;并在整体水平上采用家兔角膜血管新生模型,于缝线后第3d用地非三唑于结膜囊注射给药,每天观察角膜血管新生情况.结果 地非三唑能有效抑制人脐静脉EVC304细胞的增殖,其IC50根据药物作用时间的不同,维持在9～15μg·mL-1;并能使CAM新生血管明显减少甚至消失,对家兔角膜新生血管也有同样抑制作用.结论 地非三唑能抑制血管新生作用.OBJECTIVE To investigate the effects of antiangiogenesis of DL111-IT in vivo and in vitro.METHODS To detect the inhibition ratio of DL111-IT on endothelial cells with the method of MTT.The different concentrations of DL111-IT were injected onto the 6th day chick embryo chorioallantoic membrane(CAM),and antiangiogensis activities were assayed 72h later.Use suturation on cornea to induce the vascularization.On the third day after suturation,the rabbits were divided into four groups and gave drugs of different concentrations.The growth of neogenetic vessels of each eye was carefully observed daily during the following 21 days after suturation. RESULTS DL111-IT could inhibit the growth of neogenetic vessels effectively,IC50 of endothelial cells were ranged from 9 μg·mL-1 to 15 μg·mL-1 according to different time of drug action.The number of neogenetic vessels on the CAM were declined and it also showed the same inhibition effect on the rabbit cornea.CONCLUSION DL111-IT could inhibit angiogenesis.

关 键 词：血管新生 地非三唑 鸡胚尿囊膜 家兔角膜 

分 类 号：R972[医药卫生—药品]