高效液相色谱法测定大豆异黄酮粉中大豆苷和染料木苷的含量  

Determination of Daidzein and Genistin in Soybean Isoflavone Flour by HPLC

作　　者：李淑云[1] 赵福芹[1] 李月雄[1] 

机构地区：[1]黑龙江省医院,哈尔滨150000

出　　处：《中国现代应用药学》2007年第z2期716-717,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 探讨高效液相色谱法(High performance liquid chromatography,HPLC)测定大豆苷和染料木苷的含量.方法 十八烷基键合硅胶柱,流动相为甲醇-水-乙酸(40∶60∶1.2),检测波长254 nm.结果 大豆苷在0.008～0.4 μg内,染料木苷在0.0068～0.34 μg内,峰面积与浓度线性关系良好(大豆苷r=0.998 6;染料木苷r=0.999 9).结论 该方法简便快捷,重复性好,可作为质量控制指标之一.OBJECTIVE To establish HPLC method for the determination of Daidzein and Genistin in soybean.METHODS C18 column,The mobile phase was composed of methanol-water-acetic acid(40∶60∶1.2)with detection wavelength at 254 nm. RESULTS It is a good linear relation of the linear range and the concentration of Daidzein within 0.008～0.4 μg and the concentration of Genistin within 0.0068~0.34 μg.CONCLUSION This method was convenient,rapid and reproducible,and suitable for the quality control of the determination of Daidzein and Genistin in soybean.

关 键 词：大豆苷 染料木苷 高效液相色谱法 含量 

分 类 号：R931.6[医药卫生—生药学]