液相色谱-串联质谱法测定人血浆中左炔诺孕酮  

Determination of Levonorgestrel in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：刘飞[1] 许毓[1] 胡万群[1] 郭庆祥[1] 

机构地区：[1]中国科学技术大学化学系,安徽合肥230026

出　　处：《分析测试学报》2007年第z1期30-31,共2页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20332020)

摘　　要：A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated to quantify levonorgestrel in human plasma.After liquid-liquid extraction with ethyl acetate,the analytes were separated on a Phenomenex Luna C18 column interfaced with a tripe-quadrupole tandem mass spectrometer using positive electrospray ionization.Norethindrone was used as the internal standard.The standard calibration curve was linear over the concentration range 0.625-40 ng·mL-1.The intra-day and inter-day precisions were less than 8.16% from the determination of QC samples.Accuracy was within ±7.3%.The method was successfully applied for pharmacokinetic study of levonorgestrel after oral administration of 1.5 mg levonorgestrel to 20 healthy volunteers.A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated to quantify levonorgestrel in human plasma.After liquid-liquid extraction with ethyl acetate,the analytes were separated on a Phenomenex Luna C18 column interfaced with a tripe-quadrupole tandem mass spectrometer using positive electrospray ionization.Norethindrone was used as the internal standard.The standard calibration curve was linear over the concentration range 0.625-40 ng·mL-1.The intra-day and inter-day precisions were less than 8.16% from the determination of QC samples.Accuracy was within ±7.3%.The method was successfully applied for pharmacokinetic study of levonorgestrel after oral administration of 1.5 mg levonorgestrel to 20 healthy volunteers.

关 键 词：LEVONORGESTREL Human plasmal Liquid chromatographyl Mass spectrometry 

分 类 号：O657-55[理学—分析化学]