猪骨骼肌卫星细胞体外培养  

Culture of Porcine Myogenic Satellite Cells

作　　者：李冲[1] 刘娣 丁大有[1] 毛冠平[2] 周佳勃[2] 

机构地区：[1]东北农业大学动物科学与技术学院,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《吉林农业科学》2007年第4期52-55,共4页Journal of　Jilin Agricultural Sciences

摘　　要：探索和建立猪骨骼肌卫星细胞体外分离、培养及鉴定的技术方法。结果表明,通过机械法与酶消化法相结合的方法,15%血清完全培养液分离、培养细胞效果最好,对培养细胞进行了形态观察、生长曲线测定、染色体分析。结果表明,培养的细胞具有正常的大小、形态和染色体数目,该方法所获得的猪骨骼卫星细胞可在体外稳定培养,为有关实验提供充足的猪骨骼卫星细胞。The experiment was carried out for searching and establishing the method of culturing of myogenic satellite cells of porcine.Results demonstrated that myogenic satellite cells derived from porcine were successfully cultured with a normal tissue culture combining with enzyme digesting method and DMEM of 15% serum.The cultured cells were analyzed by morphology,chromosomes and cell growth curve.These cultured cells were typical myogenic satellite cells with normal morphology and chromosomes number.The culture and characterization of myogenic satellite cells can be provided for related experiments.

关 键 词：猪 骨骼肌卫星细胞 细胞培养 

分 类 号：S828[农业科学—畜牧学]