长俊木瓜芽增殖最适茎长的确定及壮苗培养研究  

The Selection on the Best Length for the Bud Propagation and Shoot Length Growth of Chaenomeles speciosa(Sweet) Nakai C.Lagenaria(Changjun)

作　　者：郭有燕[1] 陈明绪[2] 董丽芬[1] 贾彩霞[1] 李胜奇[3] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]陇县固关林场,陕西陇县721200 [3]商洛学院,陕西商洛726000

出　　处：《西北林学院学报》2007年第6期72-73,共2页Journal of Northwest Forestry University

摘　　要：以长俊木瓜茎段为外植体研究了长俊木瓜茎增殖的最适茎长IBA、GA3对壮苗培养的影响。结果表明:茎长≤1.0 cm的芽不利于继代,适宜于继代增殖培养的芽茎长≥1.5 cm;培养基MS+IBA(0.1 mg.L-1)+GA3(0.5 mg.L-1)+0.1%活性碳+蔗糖30 g.L-1+琼脂6 g.L-1最有利于壮苗培养。Stems of Chaenomeles speciosa were adopted as the explants to unduce buds.The suitable length of the stem was investigated.The effects of IBA and GA3 on the bud propagation were studied.The results showed that the stems less than 1.0 cm were not suitable to the subculture.And the preferable length of the stem should be more than 1.5 cm.The suitable medium for shoot growth was MS medium supplemented with 0.1 mg·L-1 IBA,0.5 mg·L-1 GA3,0.1% activated carbon,sucrose 30 g·L1 and sugar 6 g·L-1.

关 键 词：长俊木瓜 茎长 芽 壮苗培养 

分 类 号：S722.37[农业科学—林木遗传育种]