利用参比型SPR生物传感器进行SARS病毒抗原检测的研究  

Research on the Detection of SARS-CoV Antigen with Reference SPR Biosensor

作　　者：王于杰[1] 崔大付[1] 邓巍[2] 秦川[2] 王军波[1] 蔡浩原[1] 关昕[1] 王利[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室,北京100080 [2]协和医科大学中国医学科学院实验动物研究所,北京100021

出　　处：《传感技术学报》2007年第10期2151-2154,共4页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助(60341005;60427001;60501020);国家高技术研究发展计划(863)项目支持(2006AA04Z355)

摘　　要：参比型SPR生物传感器是一种可以在单通道内实现对检测、参比两个位点同时检测的新型SPR分析系统.利用参比型SPR生物传感器在线制备了两种SARS病毒抗原的检测芯片.直接固定抗体法制备的检测芯片在检测时基本无反应;蛋白A固定抗体法制备的芯片在通入灭活SARS病毒时检测信号明显,参比信号略有上升.第二种芯片可以直接检测到病毒培养液上清中的灭活SARS病毒,检测灵敏度已达到1.66×104PFU/mL.参比位点可以用来检测待测物中杂质引起的非特异性吸附.Reference SPR (Surface Plasmon Resonance) biosensor is a new sensor which can monitor reference and reaction spots in a single flow cell at same time. Two kinds of reference biochip were made on-line to detect SARS-CoV antigen. On one chip antibody to SARS-CoV was immobilized to the surface by direct coupling. On the other chip antibody to SARS-CoV was immobilized to the surface via protein A which had coupled to the chip directly. There was tiny signal change when the former was used to detect SARS-CoV antigen. While the detection signal of the latter was visible, the change of reference signal was a little. The latter chip can be used to detect sterilized SARS-CoV in culture directly. The sensitivity of SARS-CoV detection had reached to 1.66×104 PFU/mL. Reference spot can be used to detect absorption of impurity in analyte.

关 键 词：生物传感器 SARS病毒 检测 参比 SPR 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]