利用电化学生物传感器进行无创血糖检测技术研究  

Noninvasive Detection of Blood Glucose by Electrochemical Biosensor

作　　者：刘剑[1] 刘红敏[1] 杨庆德[1] 姜利英[1] 田青[1] 蔡新霞[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室

出　　处：《传感技术学报》2007年第12期2535-2539,共5页Chinese Journal of Sensors and Actuators

基　　金：国家高技术研究发展计划(863计划)资助项目(2007AA042105)

摘　　要：研制了一种新型的基于反离子电渗透原理无创检测血糖的电化学生物传感器,利用此生物传感器在自行搭建的实验装置上进行了裸鼠离体皮肤的葡萄糖透皮抽取和检测实验,研究了化学促渗剂和电流切换技术对无创血糖检测的影响.实验结果显示,使用化学促渗剂后葡萄糖的渗透量是不使用促渗剂情况下的2.29倍,采用电流切换技术,能减缓长时间通电对皮肤的刺激,在皮下葡萄糖浓度0～18mmol/L范围内,生物传感器的响应电流与皮下葡萄糖浓度具有较好的相关性(R=0.9886,n=7).此外,还进行了与生物传感器配合使用的腕式仪表的研制,为实现糖尿病人血糖闭环控制奠定技术基础.We described a novel electrochemical biosensor,with the ultimate aim to develop noninvasive detecting blood glucose based on reverse iontophoresis.Transdermal extraction and detection experiments by the biosensor had been evaluated on one homemade device using naked mouse skin in vitro.The addition of EDTA-2Na as the chemical enhancer and switching technique in which the polarity of extraction electrodes was reversed periodically were studied for the aim of extraction enhancement and avoidance of irritation on skin.Our results represented that the use of chemical enhancer produced up to 2.29-fold penetration relative to absence of it.The response of biosensor had a good correlation with the subcutaneous glucose concentration in a range of 0~18 mmol/L by the switching technique(r=0.9886,n=7).In addition,a wrist-worn device cooperated the biosensor was developed to form a key component of a closed-loop control of blood glucose level for patients with diabetes.

关 键 词：电化学生物传感器 血糖 无创检测 反离子电渗透 金电极 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]