银杏叶提取物对体外培养视网膜色素上皮细胞凋亡的影响  

Effects of Ginkgo biloba extract(EGb 761)on apoptosis of human retinal pigment epithelium cells

作　　者：项楠[1] 李贵刚[1] 王志涛[1] 罗爱珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,湖北武汉430030

出　　处：《中国现代医学杂志》2007年第8期929-932,共4页China Journal of Modern Medicine

基　　金：湖北省科技攻关基金(2004AA301C50)

摘　　要：目的研究银杏叶提取物(EGb761)对体外培养的人视网膜色素上皮细胞hRPE细胞凋亡的影响,探讨银杏叶提取物在糖尿病视网膜病变防治中的应用价值。方法体外培养人视网膜色素上皮细胞(hRPE),分别设立阴性对照(正常培养)组,阳性对照组和EGb761处理组,EGb761浓度分别为0.1、0.5和1.0g/L。首先采用浓度500microM双氧水(H2O2)作用2h诱导细胞凋亡,再分别加入不同浓度EGb761,作用24h后采用流式细胞仪、TURNAL染色法检测细胞凋亡,比较银杏叶提取物作用组与对照组间细胞凋亡率的差异。实验结果采用SPSS12.0软件统计分析。结果阴性对照组hRPE未检测到凋亡细胞,阳性对照组(H2O2作用2h)hRPE细胞凋亡率为(18.66±3.62)%;EGb761浓度分别为0.1、0.5和1.0g/L组hRPE细胞凋亡率为(14.00±1.98)%,(9.82±1.40)%,(4.94±2.06)%,组间差异有显著性(P<0.01)。结论EGb761减少H2O2诱导的人视网膜色素上皮细胞的凋亡,作用强度与药物浓度正相关,EGb761可能通过减少网膜色素上皮细胞的凋亡而减少糖尿病性视网膜病变的发生。[Objective] To investigate the effect of Ginkgo biloba extract(EGb 761)on the apoptosis of human retinal pigment epithelium cells(RPE)deduced by H2O2.[Methods] RPE cells were cultured from accidently died normal peoples.H2O2 of 500microM concentration were used to induce apoptosis before different concentration of EGb 761,which was added to test the difference of apoptosis rate among EGb 761 and control groups.Flow cytometry and TURNAL staining methods were used to detect cell apoptosis.The data were analyzed with SPSS 11.0 software.[Results] There were no RPE cells apoptosis happened in normal control group,while in positive control group(18.66±3.62)percent of RPE cells developed apoptosis,the apoptosis rate of EGb 761 groups was significantly lower than that of negative control group,which were(14.00±1.98),(9.82±1.40)%,(4.94±2.06)%(P <0.01)respectively.[Conclusions] EGb 761 can protect hRPE cells from oxidation injury induced by H2O2 in vitro.EGb 761 may be used to protect hRPE cells of diabetic patients in vivo,thus reduce complications.

关 键 词：银杏叶提取物 视网膜色素上皮细胞 糖尿病视网膜病变 

分 类 号：R961[医药卫生—药理学]