胸腺内注射同种异体抗原抑制坐骨神经移植排斥反应的免疫学研究  

Immunological study on sciatic nerve transplant tolerance induced by intrathymic injection of allogenic antigen

作　　者：高淑英[1] 张凤蕴[2] 李百祥[1] 王丽群[2] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150081 [2]哈尔滨医科大学免疫教研室,哈尔滨150081

出　　处：《中国免疫学杂志》2007年第10期916-919,共4页Chinese Journal of Immunology

基　　金：黑龙江省自然科学基金(D00-54)

摘　　要：目的:探讨胸腺内注射同种异体MHC抗原对同种异体坐骨神经移植的效应。方法:以C57BL/6(H-2b)为供体,BALB/c(H-2d)为受体,将受体鼠分为4组:Ⅰ:自体移植组、Ⅱ:异体移植组、Ⅲ:异体移植加用免疫抑制剂组、Ⅳ:胸腺内注射组:坐骨神经移植前2周将供体MHC抗原提取物注射到受体鼠的胸腺内,移植后3周进行IL-2R、TNF-α、MLR、细胞凋亡等免疫学的检测。结果:胸腺内注射组小鼠的各项免疫指标与异体移植组相比具有统计学意义。结论:胸腺内注射同种异体抗原对抑制同种异体神经移植排斥反应具有一定作用。Objective:To investigate the effect of intrathymic injection of allogenic antigen to sciatic nerve transplantation.Methods:C57BL/6(H-2b) mice were used as donors and BALB/c(H-2d) as recipients. The recipients were divided into four groups: auto-transplantation group, allogenic transplantation group, allogenic transplantation and using immunosuppressive drugs,intrathymic injection group: 3 mol/L KCl MHC antigen extractions were injected into the recipients’ thymus before two weeks before the sciatic nerves were transplanted. In the third week all the recipients underwent immunological detections for IL-2R,TNF-α,mixed lymphocytes culture and apoptosis.Results:All the detections indicated that it was of significant difference between intrathymic injection group and those in allogenic transplantation group.Conclusion:The immunological rejection of allogenic peripheral nerve transplantation can be somewhat inhibited by intrathymic injection of allogenic MHC antigen.

关 键 词：胸腺内注射 坐骨神经移植 免疫排斥 

分 类 号：R392.4[医药卫生—免疫学]