轮状病毒脂质体DNA疫苗体内诱导免疫应答研究  

Induction of immune responses against rotavirus with liposome coated rotavirus DNA vaccine

作　　者：张静[1] 王宁遂[1] 邓兵[1] 朱静[1] 

机构地区：[1]重庆医科大学附属儿童医院,重庆400014

出　　处：《中国免疫学杂志》2007年第10期928-931,共4页Chinese Journal of Immunology

基　　金：重庆市卫生局科研基金资助项目(99-2004)

摘　　要：目的:通过对轮状病毒脂质体DNA疫苗体内免疫应答研究,寻找轮状病毒DNA疫苗理想的免疫佐剂。方法:脂质体包被的轮状病毒基因疫苗pcDNA1/VP7及裸DNA经肌肉注射及鼻粘膜两种途径免疫BALB/c小鼠,利用ELISA方法对其诱导产生的体液免疫应答进行测定。结果:经裸DNA及脂质体包被的质粒DNA免疫小鼠后,滴鼻组和肌注组血清特异性IgG水平较对照组明显升高。值得注意的是,脂质体包被的质粒DNA经肌注接种后血清特异性IgA水平也较对照组明显升高,而其余各实验组均未能诱导血清特异性IgA水平的升高。结论:脂质体在DNA接种中不仅作为质粒DNA疫苗的载体,而且起到免疫佐剂的作用。经该脂质体包被的质粒DNA免疫小鼠后,肌注组血清IgA水平较对照组明显升高,提示该脂质体包被的DNA经肌肉注射途径接种可能对机体产生免疫保护。Objective:To search for the efficient immune adjuvant for rotavirus DNA vaccine by evaluating the efficacy in modifying immune responses to rotavirus.Methods:BALB/c mice were immunized by intramuscular and intranasal routes with 3 doses of naked DNA and liposome-coated DNA vaccine at two-week intervals. Titers of serum IgG and IgA were measured by ELISA.Results:Serum titers of rotavirus specific IgG in naked DNA and liposome-coated DNA vaccine-immunized mice were significantly higher than that of the unimmunized controls. Interestingly, only those mice administrated with liposome-coated DNA vaccine by intramuscular route generated serum rotavirus IgA,and neither by naked plasmid nor by intranasal immunization with DNA-liposome complex.Conclusion:These results indicate that liposome can act as both the vector and adjuvant for DNA vaccine against rotavirus by intramuscular injection,which induces rotavirus-specific IgA in the sera,and may provide partial protection against rotavirus infection.

关 键 词：轮状病毒 DNA疫苗 脂质体 免疫佐剂 粘膜免疫 

分 类 号：R373[医药卫生—病原生物学]