细粒棘球绦虫重组BCG-Eg95疫苗对感染小鼠脾细胞凋亡的影响  

The reduced apoptosis of spleen cells in infected mice by immunization with recombinant BCG-Eg95 vaccine against Echinococcus granulosus

作　　者：李文桂[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国免疫学杂志》2007年第12期1071-1073,1082,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(30671835;30500423和30200239)资助项目

摘　　要：目的:探讨细粒棘球绦虫重组BCG-Eg95疫苗免疫和Eg原头节攻击后小鼠脾细胞凋亡的变化。方法:将细粒棘球绦虫重组BCG-Eg95疫苗采用皮下注射、鼻腔内接种、口服灌胃和肌肉注射4种途径分别免疫BALB/c鼠,免疫后8周用Eg原头节进行攻击感染,感染后18周杀鼠取脾,分离脾细胞,流式细胞仪检测脾细胞的凋亡发生率,同时设有BCG和PBS对照。结果:疫苗接种组的脾细胞凋亡发生率明显低于感染对照组;口服和肌肉注射组的脾细胞凋亡发生率显著低于皮下和鼻腔内接种组。结论:Eg原头节感染可引起小鼠脾细胞发生凋亡,细粒棘球绦虫重组BCG-Eg95疫苗接种可抑制感染鼠脾细胞的凋亡,疫苗口服和肌肉注射是两种较好的免疫途径。Objective:To investigate apoptosis of spleen cells in infected mice by immunization with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg) and against challenge with Eg protoscoleces.Methods:BALB/c mice were vaccinated with the vaccine subcutaneously,intranasally,orally and intramuscularly respectively.The mice were then challenged with Eg protoscolexes at 8w of vaccination and sacrificed in 18w of infection to get spleen.Spleen cells were separated to measure apoptotic rate by FACsort with BCG and PBS served as control.Results:Apoptotic rate in the immunization group was lower than that in the control.Apoptotic rates in the oral or intramuscular group were significantly lower than that in the subcutaneous or intranasal group.Conclusion:Apoptosis of spleen cells in mice may be induced by infection with hydatid cyst,but is inhibited by immunization with rBCG-Eg95 vaccine.Oral or intramuscular vaccination may be the good regimen.

关 键 词：细粒棘球绦虫 重组BCG-EG95疫苗 脾细胞 凋亡 

分 类 号：R383.3[医药卫生—医学寄生虫学]