系统性红斑狼疮患者外周血Th2型细胞因子受体水平的研究  

Levels of Th2 cytokine receptors in the peripheral blood mononuclear cells of the patients with lupus erythematosus and its clinical significance

作　　者：姚煦[1] 李遇梅[1] 李文忠[1] 陈志强[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所,南京210042

出　　处：《中国免疫学杂志》2007年第12期1124-1128,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(30170863)资助

摘　　要：目的:探讨Th2型细胞因子受体(IL-4R、IL-6R、IL-10R)在系统性红斑狼疮(SLE)发病机制中的作用及其临床意义。方法:应用逆转录酶-聚合酶链反应(RT-PCR)检测89例SLE患者和30例正常人外周血单核细胞(PBMC)中IL-4R、IL-6R和IL-10R mRNA的表达水平;用ELISA法检测血清中IL-4、IL-6和IL-10水平。结果:①活动期SLE患者和非活动期SLE患者及正常人Th2型细胞因子受体阳性表达率为100%。②PBMC中,IL-4R mRNA表达水平:活动期SLE患者(1组)为0.604±0.147,非活动期SLE患者(2组)为0.40±0.13,正常人(3组)为0.37±0.07;IL-6R mRNA表达水平:1组为0.90±0.27,2组为0.52±0.11,3组为0.57±0.24;IL-10R mRNA表达水平:1组为0.87±0.29,2组为0.72±0.21,3组为0.68±0.14。1组和2组间比较无显著性差异(P<0.05),1组和3组间比较有显著性差异(P=0.00),2组和3组间比较无显著性差异(P>0.05)。③血清中IL-4、IL-6和IL-10水平:1组显著高于2组和3组(P<0.05),2组显著高于3组(P<0.05)。活动期SLE患者和非活动期SLE患者血清中的IL-4水平与PBMC上的IL-4R的表达水平呈正相关(r=0.622和r=0.859,P<0.05)。活动期SLE患者和非活动期SLE患者血清中的IL-6水平与PBMC上的IL-6R的表达水平呈正相关(r=0.887和r=0.615,P<0.05)。活动期SLE患者和非活动期SLE患者血清中的IL-10水平与PBMC上的IL-10R的表达水平呈正相关(r=0.888和r=0.787,P<0.05)。结论:Th2型细胞因子及其受体的异常表达可能在SLE疾病活动和发展过程中起重要作用。检测SLE患者PBMC中Th2型细胞因子受体的表达水平可作为疾病的活动性指标之一。Objective:To investigate the roles of Th2 cytokine receptors in the pathogenesis of systemic lupus erythematosus(SLE) and its clinical significance.Methods:RT-PCR technique was used to semiquantitatively analyze Th2 cytokine receptors mRNA expression in PBMC from 89 patients with SLE,double antibody sandwich ELISA was applied to determine Th2 cytokines in serum.Results:The positive Th2 cytokine receptors mRNA expression accounted for 100% among active SLE group(group Ⅰ),inactive SLE group(group Ⅱ) and control group(group Ⅲ).The mean levels of IL-4R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.604±0.147,0.40±0.13 and 0.37±0.07,respecitively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-4R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.351).The mean levels of IL-6R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.902±0.273,0.519±0.11 and 0.573±0.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.289).The mean levels of IL-10R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.87±0.29,0.722±0.209 and 0.684±0.138,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.405).The mean levels of IL-4 in serum in group Ⅰ,Ⅱ and Ⅲ were 105.57±29.17,51.17±16.69 and 23.73±6.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL(4 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-6 in serum in group Ⅰ,Ⅱ and Ⅲ were 71.89±20.02,17.96±6.93 and 0.035±0.035,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6 in group

关 键 词：系统性红斑狼疮 IL-4 IL-4受体 IL-6 IL-6受体 IL-10 IL-10受体 

分 类 号：R392[医药卫生—免疫学]