GSK-3及P70S6K在胰岛素抵抗大鼠肌肉中的表达及意义  

Expression and significance of GSK-3 and P70S6K in rat muscles with insulin resistance

作　　者：王艳军[1] 赵晟[1] 刘国良[2] 

机构地区：[1]中国医科大学附属盛京医院内分泌科,沈阳110004 [2]中国医科大学第一临床学院内分泌科,沈阳110001

出　　处：《中国免疫学杂志》2007年第12期1133-1135,共3页Chinese Journal of Immunology

基　　金：辽宁省教育厅课题(No2004D166)资助

摘　　要：目的:观察饮食诱导的胰岛素抵抗(IR)大鼠骨骼肌中糖原合成酶-3(GSK-3)及核糖体S6蛋白激酶(P70S6K)的表达及变化情况,并探讨GSK-3和P70S6K在IR发生中的作用及意义。方法:将40只4周龄Wistar大鼠随机分为正常对照组、高糖组、高脂组、高脂高糖饲养组,喂养8周后用高胰岛素-正葡萄糖钳夹技术(钳夹试验)对大鼠进行胰岛素敏感性的评估,采用Westernblot法检测各组大鼠骨骼肌中GSK-3及P70S6K的含量,同时测定各组大鼠的附睾脂肪垫重量、血糖(BG)、胰岛素(INS)、甘油三酯(TG)、胆固醇(TC)及游离脂肪酸(FFA)水平、超敏C反应蛋白(hsCRP)。结果:高脂高糖组、高脂组大鼠产生明显IR,体重增加(P<0.01),以附睾脂肪垫的重量增加更为显著(P<0.01),TG、TC及FFA水平、hsCRP增加(P<0.01),GSK-3、P70S6K在IR大鼠肌肉中的表达明显升高。结论:高脂高糖饮食可诱导大鼠产生IR,IR大鼠的hsCRP、TG、TC及FFA水平明显增高,大鼠产生IR与炎症反应有关,GSK-3、P70S6K在IR大鼠中的表达明显升高,在胰岛素信号传导中起负相调节作用。Objective:To observe expression and diversity of glycogen synthase (GSK)-3 and ribosome S6 protein kinase (P70S6K) in skeletal muscle of insulin resistance (IR) rat induced by high fat food and to approach the effect and significance of GSK-3 and P70S6K in IR occurrence.Methods:Forty Wistar rats aged four-week were randomly divided into normal control, high-glucose, high-fat and high-fat high-glucose groups. 8 weeks after feeding, insulin sensitivity of the rats was evaluated by hyperinsulinism euglycaemic clamps technique (ring clamp experiment), GSK-3 and P70S6K in rat skeletal muscle were measured by Western blot and epididymis fat pad weight, blood glucose, insulin, triglyceride (TG), cholesterin (TC), free fatty acids (FFAs) and hsCRP levels were also determined.Results:IR was induced, body weight (P<0.01)especially of epididymal fat pad (P<0.01) elevated FFA, TG, TC and hsCRP levels increased (P<0.01), and the expressions of GSK-3 and P70S6K were enhanced in muscle of high-fat and high-fat high-glucose groups.Conclusion:High-fat high-glucose diet could induce rat IR with increased serum hsCRP, TG, TC and FFA levels. IR could be correlated with inflammation reactions in rats. The enhanced GSK-3 and P70S6K expressions in IR rats might play a role in the negative regulation of insulin signal transduction.

关 键 词：糖原合成酶3(GSK-3) 核糖体S6蛋白激酶(P70S6K) 胰岛素抵抗(IR) 信号传导 

分 类 号：R587.3[医药卫生—内分泌]