杜仲药材高效液相色谱指纹图谱研究  

Study on fingerprint of Eucommia ulmoides by HPLC

作　　者：黄平[1] 席先蓉[1] 张琼[2] 张恩景[2] 韩双[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]湖北中医学院,湖北武汉430065

出　　处：《齐鲁药事》2007年第11期660-662,共3页qilu pharmaceutical affairs

摘　　要：目的应用高效液相色谱法对不同产地杜仲药材进行指纹图谱研究。方法XDB-C18色谱柱(250mm×4.6mm,5μm),乙腈-1%乙酸溶液二元梯度洗脱,体积流量1mL.min-1,检测波长277nm。结果根据选择的色谱条件测定了不同产地杜仲药材的指纹图谱。结论该方法操作简便,稳定,重复性好,可为更好地控制杜仲药材的内在质量提供科学依据。OBJECTIVE To study the fingerprint of raw material of Eucommia ulmoides Oliv.from various habitats by HPLC.METHODS XDB-C18 column(250mm×4.6mm,5μm)was used,with mixtures of acetonitrile and 1% acetic acid as mobile phase in a gradient mode.The flow rate was 1mL·min-1 and detection wavelength was 277nm.RESULTS According to the optimum chromatographic conditions,good fingerprint of Eucommia ulmoides Oliv.from different habitats had been described.CONCLUSION The method was simple and accurate with good reproducibility.It provides a scientific basis for controlling the quality of Eucommia ulmoides Oliv..

关 键 词：杜仲 高效液相色谱法 指纹图谱 

分 类 号：R284[医药卫生—中药学]