心脑治胶囊中人参皂苷Rg_1、Rb_1及三七皂苷R_1的检测方法  

Study on determination method of Ginsenoside Rg_1,Rb_1 and Notoginsenoside R_1 in Xinnaozhi Capsules

作　　者：白保国[1] 

机构地区：[1]济宁市第二人民医院,山东济宁272000

出　　处：《齐鲁药事》2007年第11期665-667,共3页qilu pharmaceutical affairs

摘　　要：目的 建立心脑治胶囊中人参皂苷及三七皂苷含量的检测方法.方法 采用高效液相色谱法,色谱柱为Zorbax C18柱,乙腈-水梯度洗脱(0～3min,20%～25%乙腈;3～15min,25%～45%乙腈);流速1.0mL·min-1;检测波长203nm.结果 人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1线性关系良好(r≥0.999),平均回收率(n=5)分别为105.68%(RSD=1.52%)、 104.34%(RSD=0.73%)、102.93%(RSD=1.26%).结论 本含量测定方法灵敏、简便、快速、准确,适用于心脑治胶囊内在质量的控制.OBJECTIVE To estibish an HPLC method to determine Xinnaozhi Capsules,ginsenoside and the notoginsenoside in Xinnaozhi Capsules.METHODS Zorbax C18 column with gradient elution was adopted.Solvent A was acetonitrile and solvent B was water.The gradient program of solvent A was as follows:0～3min,20%～25%;3～15min,25%～45%.The flow rate was 1.0mL·min-1,the detection wavelength was 203nm.RESULTS Ginsenoside Rg1,ginsenosideRb1 and notoginsenoside R1 linearity relation were fine(r≥0.999),average coefficient of recovery(n=5) was 105.68%,(RSD=1.52%),104.34%(RSD=0.7%),102.93%(RSD= 1.26%),respectively.CONCLUSION The method was sensitive,simple,accurate and suitable for the quality control of Xinnaozhi Granules.

关 键 词：心脑治胶囊 人参皂苷 三七皂苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]