A developmental biological study of aldolase gene expression in Xenopus laevis  

作　　者：KOICHIRO SHIOKAWA, ERI KAJITA, HIROSHI HARA, HITOMI YATSUKI, KATSUJI HORI1 Laboratory of Molecular Embryology, Department of Biological Sciences, Graduate School of Science, TheUniversity of Tokyo, Hongo 7-3-1, Bunkyo ku, Tokyo 113-0033, Japan 2Medical Re 

出　　处：《Cell Research》2002年第2期85-96,共12页细胞研究（英文版）

摘　　要：We cloned cDNAs for Xenopus aldolases A, B and C. These three aldolase genes are localized on different chromosomes as a single copy gene. In the adult, the aldolase A gene is expressed extensively in muscle tissues, whereas the aldolase B gene is expressed strongly in kidney, liver, stomach and intestine, while the aldolase C gene is expressed in brain, heart and ovary. In oocytes aldolase A and C mRNAs, but not aldolase B mRNA, are extensively transcribed. Thus, aldolase A and C mRNAs, but not B mRNA, occur abundantly in eggs as maternal mRNAs, and strong expression of aldolase B mRNA is seen only after the late neurula stage. We conclude that aldolase A and C mRNAs are major aldolase mRNAs in early stages of Xenopus embryogenesis which proceeds utilizing yolk as the only energy source, aldolase B mRNA, on the other hand, is expressed only later in development in tissues which are required for dietary fructose metabolism.We also isolated the Xenopus aldolase C genomic gene (ca. 12 kb) and found that its promoter (ca. 2 kb)contains regions necessary for tissue-specific expression and also a GC rich region which is essential for basal transcriptional activity.

关 键 词：Amino Acid Sequence Animals Cloning  Molecular DNA  Complementary Fructose-Bisphosphate Aldolase Models  Anatomic Models  Genetic Models  Molecular Molecular Sequence Data RNA  Messenger Sequence Homology  Amino Acid Time Factors Tissue Distribution Xenopus laevis 

分 类 号：Q344.13[生物学—遗传学]