CD4^+CD25^+调节性T细胞对狼疮样小鼠的干预性研究  

Reverse effect of CD4^+CD25^+ regulatory T cells on lupus-like murine

作　　者：苏虹[1] 王保龙[2] 方雪晖[1] 陈娟[1] 叶冬青[1] 

机构地区：[1]安徽医科大学公共卫生学院流行病与卫生统计学系,安徽合肥230032 [2]安徽省立医院输血科,安徽合肥230001

出　　处：《疾病控制杂志》2008年第2期147-151,共5页Chinese Journal of Disease Control and Prevention

基　　金：国家自然科学基金(30571608、30771848);安徽省自然科学基金(070413109)

摘　　要：目的分析CD4+CD25+调节性T细胞(regulatory T cells,Tregs)对狼疮样小鼠的干预效应,为系统性红斑狼疮(systemic lupus erythematosus,SLE)的免疫干预提供新的思路。方法采用磁活性标记的细胞分选方法(magnetic activated cell sorting,MACS)分选BALB/c小鼠CD4+CD25+T细胞,应用流式细胞术(flow cytometry,FCM)检测分选细胞纯度。将6~8w♀CB6F1小鼠随机分为3组,包括正常对照组、狼疮鼠模型组(亲代淋巴细胞免疫)、狼疮鼠干预组(亲代淋巴细胞免疫后2w,输入5×106Tregs/鼠)。分别于诱导后2、4、8、12w,采用ELISA法检测ANA和抗-dsD-NA抗体,并观察肾标本的病理学改变。结果BALB/c小鼠脾脏单个核细胞经MACS分选后,CD4+CD25+T细胞纯度达98.5%。经亲代淋巴细胞输注后,CB6F1小鼠出现体重降低、皮肤干涩;ANA和抗ds-DNA抗体上升;以及狼疮肾炎的病理学改变。而输入Tregs对已出现自身抗体的狼疮鼠有明显的逆转效应,CB6F1小鼠于输注后抗-dsDNA即开始下降,并伴随其他临床症状和实验室指标的逐渐改善。结论CD4+CD25+Tregs具有明显的逆转CB6F1鼠狼疮样疾病的效应,将为SLE的免疫干预开辟新途径。Objective To analyze the reverse effect of CD4+CD25+ regulatory T cells(Tregs)on lupus-like murine,so as to provide a new immunotherapy for systemic lupus erythematosus(SLE).Methods CD4+CD25+T cells of BALB/c murine were separated with MACS and the purity was analyzed by FCM.6-8 wk female CB6F1 mice were selected randomly into three groups(five mice per group).There were normal control,lupus-like murine(transfer of parental splenic lymphocytes)and intervention group(transfer of Tregs at days 14 after induct...

关 键 词：T淋巴细胞 红斑狼疮 系统性 

分 类 号：R593.241[医药卫生—内科学]