IFN-γ促进人单核细胞向不典型成熟DC分化  

IFN-γ promotes human monocytes to differentiate to untypical mature DC

作　　者：王惠明[1] 王沂芹[2] 阮志华[2] 傅晓岚[2] 徐文岳[2] 赵婷婷[2] 吴玉章[2] 

机构地区：[1]第三军医大学大坪医院肾内科,重庆400042 [2]第三军医大学基础部全军免疫学研究所,重庆400038

出　　处：《细胞与分子免疫学杂志》2008年第3期234-237,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30400215)

摘　　要：目的:研究IFN-γ对人单核细胞表型及分化的影响。方法:采用免疫磁珠法从健康志愿者外周血单个核细胞(PBMCs)中特异性分选单核细胞,以细胞因子IFN-γ、TNF-α、IFN-α刺激单核细胞后,观察单核细胞生长特性,并以流式细胞术检测单核细胞表面CD1a、CD14、CD80、CD83、CD86、HLA-DR等分子表达。结果:细胞因子IFN-γ、TNF-α、IFN-α对单核细胞形态、生长及表型转化有不同影响。IFN-γ促进单核细胞黏附聚集形成"细胞小岛结构",刺激单核细胞形态向梭形及多角形转化;IFN-γ上调单核细胞表达CD80、CD83、CD86及HLA-DR分子,同时下调CD14分子表达。结论:IFN-γ促进单核细胞向不典型的成熟DC方向分化。AIM:To explore the morphologic and phenotypic changes of monocytess in response to the stimulation of IFN-γ,TNF-α and IFN-α.METHODS:Newly isolated monocytes from human PBMCs were treated with IFN-γ,TNF-α or IFN-α.The phenotypic growth of monocytes with or without stimulation was observed by microscope.The surface expression of CD1a,CD14,CD80,CD83,CD86,and HLA-DR on the monocytes was assayed by flow cytometry.RESULTS:After IFN-γ stimulation,monocytes displayed the shape of fusiform or polygon and were more l...

关 键 词：单核细胞 IFN-Γ 表型 

分 类 号：R392[医药卫生—免疫学]