不同副溶血弧菌的分子鉴别与生长动力学模型比较  

Molecular characterization and microbial growth kinetics model of Vibrio parahaemolyticus isolates from different sources

作　　者：杨振泉[1] 焦新安[1] 

机构地区：[1]江苏省人兽共患病学重点实验室,扬州大学扬州225009

出　　处：《中国人兽共患病学报》2008年第3期210-215,共6页Chinese Journal of Zoonoses

基　　金：国家杰出青年科学基金资助项目(30425031)

摘　　要：目的探讨不同分子亚型副溶血弧菌(Vibrio parahaemolyticus,Vp)的生长动力学特征,为建立海产品中Vp预测模型提供数据。方法应用PCR和RAPD方法对5株不同来源的Vp分离株进行了分子鉴别,并对不同菌株在适宜温度(37℃)和低温(15℃)下生长变化进行了测定,采用修正的Gompertz方程拟合细菌的生长曲线,求出不同菌株的生长动力学参数并建立预测模型。结果4株食物中毒样品来源的Vp菌株基因型均为tl+tdh+trh-toxR+,1株海产品分离株为tl+tdh-trh-toxR+,筛选的随机引物P21可以将5株不同来源的Vp菌株分成5个不同的分子亚型,不同菌株37℃的世代时间在0.210~0.238h之间,延滞时间在1.705~1.849h之间,生长曲线没有显著差异(R2>0.995),应用预测模型求得各菌株的预测值与实测值均方根偏差在0.110~0.452之间;而在15℃条件下测得不同Vp菌株世代时间在0.727~1.062h之间,延滞时间在10467~12552h之间,生长曲线具有较大的变异性(R2>0.938),其中菌株VP06003和VP06127的预测值与实测值之间均方根偏差分别为...To characterize growth kinetics model of different subtypes of Vibrio parahaemolyticus and to provide useful data for developing risk assessment of V. parahaemolyticus in seafood, PCR and RAPD techniques were applied to molecular characterization of 5 strains of V. parahaemolyticus isolated from different sources and the growth of bacterial at 37℃ and 15℃ were analyzed. Growth curves were fitted using modified Gompertz equation and kinetic parameters of different strains and predictive model were developed ...

关 键 词：副溶血弧菌 分子鉴别 生长动力学模型 预测模型 

分 类 号：R378[医药卫生—病原生物学]