华支睾吸虫溶血磷脂酶在虫体组织定位及其特异性抗体类型分析  

The histo-localization of lysophospholipase from Clonorchis sinensis and the isotype analysis for the specific antibody responses

作　　者：马长玲[1] 胡旭初[1] 胡凤玉[1] 郑小凌[1] 徐劲[1] 吕芳丽[1] 余新炳[1] 

机构地区：[1]中山大学中山医学院寄生虫学教研室

出　　处：《中国人兽共患病学报》2008年第3期220-223,共4页Chinese Journal of Zoonoses

基　　金：广东省重大科技专项(2004A30801004);2006"863"专题(2006AA02Z422)基金资助

摘　　要：目的观察华支睾吸虫溶血磷脂酶(CsLysoPLA)在成虫的组织定位,分析其诱导人体产生的特异性抗体类型。方法应用免疫荧光方法,观察CsLysoPLA在成虫的组织定位;应用ELISA方法,CsLysoPLA重组蛋白检测25份华支睾吸虫病人血清的IgG1、IgG2、IgG4、IgE。结果CsLysoPLA定位于虫体的口吸盘、排泄囊、肠支、生殖孔,与虫体分泌/排泄抗原物质密切相关的部位;以CsLysoPLA重组蛋白检测华支睾吸虫感染者血清,IgG1抗体水平较高,IgG2抗体水平稍高,而感染者的IgG4、IgE水平则与健康人无明显差异。结论CsLysoPLA主要经虫体消化道、生殖道、排泄系统分泌/排泄到体外,刺激人体免疫系统产生Th2为主的免疫应答。To observe the histo-localization of lysophospholipase from Clonorchis sinensis (CsLysoPLA) and to analyze the isotype of specific antibody responses in humans, the localization of CsLysoPLA from adult worms was demonstrated by immuno-fluorescent techniques ,and the recombinant CsLysoPLA protein was used as antigen in ELISA assay to detect the presence of the specific antibodies of IgG1, IgG2, IgG4 and IgE in serum samples of 25 patients with C. sinensis infection. It was found that CsLysoPLA was localized ...

关 键 词：华支睾吸虫 溶血磷脂酶 组织定位 抗体类别 

分 类 号：R392[医药卫生—免疫学]