TLR在小鼠胸腺中的表达及其在胸腺细胞发育中的可能作用  

Expression of TLR in mouse thymus and potential role in thymocyte development

作　　者：张凤[1] 龚燕萍[1] 徐薇[1] 储以微[1] 熊思东[1] 

机构地区：[1]复旦大学免疫生物学研究所

出　　处：《现代免疫学》2008年第1期31-36,共6页Current Immunology

基　　金：上海医学领军人才基金资助项目(LJ06011);国家自然科学基金资助项目(30671952);上海市科委国际合作项目(045407038)

摘　　要：检测体外培养和体内发育过程中,胎鼠胸腺处于不同发育阶段时Toll样受体(TLR)的表达,阐明TLR表达量与胸腺细胞发育相关性,为TLR和胸腺细胞发育分化相关研究提供基础数据。无菌取15d胎龄胎鼠胸腺进行体外培养(FTOC),在培养不同时间点(2d,4d,6d),检测处于不同发育期胸腺TLR的表达;同时在孕期不同天数(15~19d),分别取胎鼠胸腺,检测在体内发育过程中胸腺TLR的表达;在FTOC中加入二脱氧鸟苷培养6d以制备胸腺基质细胞,检测基质细胞与胸腺细胞TLR表达情况。结果:小鼠胸腺中检测到多种TLR。FTOC培养中:培养第2天(F2)开始检测到各种TLR,到培养第6天(F6),TLR1,TLR3,TLR6,TLR7,TLR8明显上调,而TLR4,TLR5保持低水平,TLR4在培养第6天又下降;体内发育过程中:TLR6表达量随胎龄增加有较明显上调,TLR1,TLR3-8保持低水平表达;TLR2,TLR9体内体外都未检测到明显表达。在对胸腺细胞与基质细胞TLR表达比较中发现TLR1,TLR5,TLR6,TLR7高表达于胸腺细胞。胎鼠胸腺表达某些TLR,并且在发育不同阶段表达量有所改变,提示TLR可能参与胸...Expressions of TLR in fetal mouse thymus were investigated both in vitro and in vivo,thus to determine the association of expression of TLR with development of thymocytes and to provide basic information for further study on TLR and thymocyte development. Thymuses of fetal mice of 15 gestation day were separated sterilely,and cultured in FTOC,and those of various gestation days were also isolated. Thymuses with different development stages,which were cultured for various days,or freshly separated from fetal...

关 键 词：TLR 表达谱 胸腺 FTOC 

分 类 号：R392[医药卫生—免疫学]