FOXP3和GITR/GITRL mRNA在类风湿关节炎和结直肠癌患者外周血中的表达研究  

Expression of FOXP3 and GITR/GITRL mRNA in peripheral blood monocytes of patients with rheumatoid arthritis and colorectal cancer

作　　者：杨蔺[1] 宗明[1] 卢添宝[1] 李红梅 韩捷[3] 王强[4] 何俊民[5] 范列英[1] 

机构地区：[1]同济大学附属上海市东方医院检验科,上海201200 [2]生物芯片上海国家工程研究中心,上海201203 [3]同济大学附属上海市东方医院免疫科,上海201200 [4]第二军医大学长征医院普外科,上海200003 [5]同济大学生命科学与技术学院,上海200092

出　　处：《现代免疫学》2008年第1期66-70,共5页Current Immunology

基　　金：上海市自然科学基金资助项目(05ZR14099)

摘　　要：探讨FOXP3和糖皮质激素诱导的肿瘤坏死因子受体(glucocorticoid-induced tumor necrosis factor receptor,GITR)及其配体(GITRL)在类风湿关节炎(rheumatoid arthritis,RA)和结直肠癌患者中的表达变化及GITR与FOXP3表达的相关性。运用实时荧光定量RT-PCR方法,检测55例健康体检者、55例RA患者、50例结直肠癌患者的外周血单个核细胞(peripheral blood monocytes,PBMC)中FOXP3、GITR和GITRL的基因表达水平。结果:在RA患者组FOXP3、GITR和GITRL均显著低于健康对照组(P<0.01),经过转换后的mRNA表达水平分别为对照组的18.5%、7.5%和1.7%,GITR和FOXP3的表达水平呈显著正相关(r=0.86,P<0.01);而在结直肠癌患者组,FOXP3的表达水平显著高于对照组(P<0.01),表达水平为对照组的173.7%,GITR和GITRL的表达水平显著低于对照组(P<0.01),mRNA表达水平为对照组的45.7%和50.1%,GITR和FOXP3的表达有一定的相关性(P=0.046)。RA和结直肠癌患者均存在调节性T细胞(regulatory Tcells,Treg)异常。在RA中,GITR表达趋势与FOXP3一致,均反映Treg数量或功能降低。但在结直肠癌患者中,两者表达不尽一致。The changes on the mRNA expression of FOXP3 and glucocorticoid-induced TNF-receptor(GITR) and its ligand(GITRL) in peripheral blood monocytes of patients with rheumatoid arthritis(RA) and colorectal cancer and their correlation were investigated in the present study, By means of real-time quantitative reverse transcription PCR(QR-RT-PCR),the expression of FOXP3 and GITR/GITRL was detected from 55 healthy individuals on physical examinations,55 patients with rheumatoid arthritis and 50 patients with colorect...

关 键 词：FOXP3 GITR GITRL 类风湿关节炎 结直肠癌 

分 类 号：R593.22[医药卫生—内科学]