米诺环素对小鼠T淋巴细胞体外活化和增殖的影响  

Effect of minocycline on the in vitro activation and proliferation of murine T lymphocytes

作　　者：姚满林[1] 曾耀英[1] 黄秀艳[1] 周建国[1] 梁兆端[1] 杨志[1] 

机构地区：[1]暨南大学组织移植与免疫中心,广州510632

出　　处：《现代免疫学》2008年第2期136-141,共6页Current Immunology

基　　金："973"国家重点基础研究项目(2006CB504201)

摘　　要：研究米诺环素(MNC)对刀豆蛋白A(ConA)刺激的小鼠T细胞体外活化和增殖的影响,并对其免疫调节作用进行初步探讨。以ConA刺激小鼠淋巴结来源的淋巴细胞,以不同终浓度的MNC与T细胞共培养,荧光抗体染色结合流式细胞术,检测T细胞早期活化抗原CD69的表达;用羧基荧光素双醋酸盐琥珀酰(CFDA-SE)染色结合流式细胞术,并用ModFit软件分析T细胞增殖相关指数。结果:终浓度为10、25和50μmol/L的MNC显著抑制ConA诱导的T细胞CD69的表达,由ConA组的71.20%±1.08%,分别降低为64.43%±1.39%、53.34%±1.26%和33.65%±1.91%;CFDA-SE染色结果显示,培养48 h和72 h的ConA组T细胞的增殖指数(PI)分别为1.57±0.03和2.06±0.02,各浓度的MNC对ConA刺激的T细胞增殖具有明显的抑制作用,以50μmol/L的MNC抑制作用最明显,48 h和72 h的PI分别为1.01±0.02和1.03±0.01,与相应时间的对照组比较,均有统计学意义(P<0.01)。MNC能有效地抑制小鼠T细胞的体外活化和增殖。具有独立于其抗菌活性的抗炎作用。In the present studiy,the effect of minocycline on the activation and proliferation of murine lymphocytes was investigated in vitro in order to elucidate its immunosuppressive mechanism,in which the T lymphocytes derived from lymph nodes of mice were stimulated with ConA and cocultured with different concentrations of minocycline.The expression of the early activated antigen CD69 on T lymphocytes was detected by fluoresent mono-antibodies and proliferation was detected by carboxyl fluorescein diacetate-succ...

关 键 词：米诺环素 CD69 细胞增殖 流式细胞术 T细胞活化 

分 类 号：R96[医药卫生—药理学]