MBL与人单核细胞系THP1/CD14细胞结合及其特性研究  

Characterization of the binding of mannan-binging lectin to human monocytoid cell line THP1/CD14

作　　者：张雅妮[1] 王明永[1] 雷鸣[1] 卢晓[1] 陈政良[1] 

机构地区：[1]南方医科大学免疫学教研室,广州510515

出　　处：《现代免疫学》2008年第2期121-125,共5页Current Immunology

基　　金：广东省自然科学基金研究团队资助项目(015003)

摘　　要：采用Cell-ELISA和流式细胞术,发现人单核细胞系THP1/CD14在生理条件下可结合甘露聚糖结合凝集素(MBL),这种结合具有Ca2+依赖性,能被甘露糖、N-乙酰葡糖胺、D-葡萄糖、半乳糖、蔗糖、海藻糖等及重组人MBL-CRD蛋白和MBL-CLR蛋白所部分抑制,但C1q或抗人C1qR单克隆抗体对之无影响。还发现,炎性状态下的THP1/CD14细胞与MBL的结合增强。结果表明,THP1/CD14细胞表达Ca2+依赖的、糖敏感的MBL受体或结合蛋白,包括对CLR特异和CRD特异的两种受体,均与C1q受体无关;炎性刺激可上调THP1/CD14细胞MBL受体或结合蛋白的表达。By means of cell-ELISA assay and flow cytometry,the human momocytoid cell line THP1/CD14 was identified and its binding to mannan-binding lectin(MBL) could be demonstrated under physiological conditions.It was found that this binding was Ca2+ ion concentration-dependent and could be partially inhibited by a variety of carbohydrates,such as mannose,GlcNAc,D-glucose,galactose,trehalose and sucrose,as well as by recombinant human MBL carbohydrate recognition domain protein(rhMBL-CRD) and recombinant human MBL ...

关 键 词：甘露聚糖结合凝集素 受体/结合蛋白 THP1/CD14细胞 

分 类 号：R392[医药卫生—免疫学]