青藤碱对胶原诱导型关节炎免疫抑制作用的机制研究  

The mechanism of immunosuppressive effect of sinominine on collagen induced arthritis of rat

作　　者：陈光星[1] 王培训[1] 刘清平[1] 童娟[1] 沈晓燕[1] 黄清春[1] 陈纪藩[1] 

机构地区：[1]广州中医药大学第一附属医院六内风湿专科,广州510405

出　　处：《现代免疫学》2008年第2期142-146,共5页Current Immunology

基　　金：国家自然科学基金重点资助项目(39930230);广东省中医药管理局课题资助项目(102019)

摘　　要：为证实青藤碱(SINO)抗关节破坏的作用,揭示其可能的作用途径,采用牛II型胶原混合弗氏不完全佐剂于Wistar大鼠尾根部注射。足肿后随机分为造模加生理盐水组、造模加雷公藤多苷(TG)治疗组和造模加SINO治疗组,容积法每天测量后肢肿胀度。给药后第35天,乳腺钼靶机大鼠全身摄片,计分法评价四肢关节骨侵蚀和关节间隙变化;取足趾关节和腹股沟淋巴结,HE染色,评价病理情况;培养原代滑膜细胞,放免法检测培养上清IL-6,RT-PCR检测IL-6 mRNA表达。结果显示,SINO和TG可明显抑制关节肿胀,骨侵蚀和关节间隙变化(P<0.01);抑制CIA大鼠滑膜增生、软骨破坏、淋巴结生发中心反应和髓索浆细胞浸润(P<0.01);降低滑膜细胞IL-6 mRNA和蛋白水平。提示SINO和TG具有抑制关节炎症和关节破坏的作用,其作用机制可能是减少IL-6的产生,抑制B细胞的激活和分化。To confirm the effect of sinominine(SINO) on joint destruction and to explore its possible mechanism of action,bovine collagen type II(CII) emulsified with Freund s incomplete adjuvant was injected intradermally at the base of tail of Wistar rats.When the swelling in the hind limb of rats occurred,they were randomly divided into 3 groups:saline control group;treatment group with SINO and treatment group with SINO + tripterygium glycoside(TG),and the swelling in the hind limbs was measured daily by cubic met...

关 键 词：胶原诱导型关节炎 青藤碱 体液免疫 IL-6 

分 类 号：R285[医药卫生—中药学]