SBR基因原核表达质粒的构建  

Construction of prokaryotic expression vector of SBR gene

作　　者：张苗[1] 陈洪雷[1] 姜广水[2] 杨丕山[2] 卞继峰[3] 

机构地区：[1]解放军第88医院,山东泰安271000 [2]山东大学口腔医学院,山东济南250012 [3]山东大学西校区分子生物学教研室,山东济南250012

出　　处：《泰山医学院学报》2008年第2期90-92,共3页Journal of Taishan Medical College

摘　　要：目的构建可用于大肠杆菌表达系统的含变形链球菌唾液结合区段(SBR)基因的表达载体pcMVT7-SBR。方法用定向克隆方法将SBR基因插入表达载体pcMVT7,构建重组原核表达质粒pcMVT7-SBR。结果经酶切鉴定及DNA序列测定,重组质粒pcMVT7-SBR序列及阅读框架正确。结论SBR表达载体构建成功,为防龋疫苗的动物实验研究奠定基础。Objective: To obtain the prokaryotic expression vector containing the saliva binding region(SBR) gene of Streptococcus mutans.Methods: By directional cloning method,SBR gene fragment was cloned into the expression vector pcMVT7 and recombinant prokaryotic expression plasmid pcMVT7-SBR with right reading frame was successfully constructed.Results: Restriction endonuclease and DNA sequencing techniques were used to identify the recombinant plasmid DNA.The results were in correspondence with the initial design...

关 键 词：唾液结合区段 构建 防龋疫苗 

分 类 号：R346[医药卫生—基础医学]