解脲脲原体的tetM基因与四环素类药物MIC水平的关联性研究  

Relationship between gene tetM of Ureaplasma urealyticum (Uu) with MIC level of tetracyclines

作　　者：亓春花[1] 

机构地区：[1]泰山医学院附属泰安医院,山东泰安271000

出　　处：《泰山医学院学报》2008年第2期84-86,共3页Journal of Taishan Medical College

基　　金：泰安市科技发展计划项目(2002-48)

摘　　要：目的探讨目前解脲脲原体对四环素类药物的耐药性及其与tetM耐药基因的关系。方法对分离自临床妇产科和性病门诊患者的解脲脲原体49株,经鉴定确认后,采用微量肉汤稀释法检测其四环素和米诺环素的最小抑菌浓度(MIC),根据药敏结果分层随机抽取部分菌株采用PCR技术扩增四环素耐药基因tetM。结果解脲脲原体的米诺环素和四环素MIC50(50%菌株被抑制所需抗生素浓度)分别为<0.0625 mg/L和<0.125 mg/L;MIC90(90%菌株被抑制所需抗生素浓度)分别为0.125 mg/L和1 mg/L;在28株非四环素耐药的解脲脲原体菌株中,有8株检测到tetM基因tetM基因阳性组的四环素和米诺环素的MIC水平比tetM阴性组高,且差异有统计学意义(四环素:t=4.34,P=0.0001;米诺环素:t=5.90,P<0.0001)。结论在泰安地区四环素类药物可以重新作为治疗解脲脲原体感染的一线药物,尤其是米诺环素的抗菌效果较好;在非四环素耐药的解脲脲原体菌株中也存在tetM基因的携带,且影响其MIC的提高。Objective: To determine the relationship between the drug resistance of tetracyclines to Ureaplasma urealyticum(Uu) and their distribution of the resistance gene tetM.Methods: In vitro susceptibility to tetracycline and minocycline of 49 strains of Uu from clinical patients was determined by broth microdilution method,the resistance gene tetM from Uu was amplified by PCR.Results: It was found that the MIC50s to tetracycle and minocycline were 〈0.0625 mg/L and 〈0.0125 mg/L respectively,and the MIC90s to thes...

关 键 词：解脲脲原体 米诺环素 四环素 最小抑菌浓度 tetM耐药基因 

分 类 号：R446.5[医药卫生—诊断学]