中华绒螯蟹、日本绒螯蟹及其杂交F_1代群体的遗传多样性AFLP分析  

AFLP Analysis in Populations of Chinese Mitten Crab(Eriocheir sinensis),Japanese Mitten Crab(E.japonica)and Their Hybrids

作　　者：董颖[1] 周遵春[1] 宋伦[1] 阎有利 

机构地区：[1]辽宁省海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室辽宁,大连116023 [2]辽宁省淡水水产科学研究院,辽宁辽阳111000

出　　处：《水产科学》2008年第3期135-138,共4页Fisheries Science

基　　金：国家908辽宁专项(LN-908-01-01-06)

摘　　要：通过4对引物组合对辽河水系中华绒螯蟹群体,俄罗斯的日本绒螯蟹群体及其杂交F1代(辽河蟹♀×俄罗斯蟹♂)的遗传多样性进行了AFLP分析。结果表明,4对引物共扩增出233条带(100bp^1000 bp),其中辽河群体多态条带184条,多态位点比例78.97%;俄罗斯群体177条多态带,多态位点比例75.97%;杂交群体182条多态带,多态位点比例78.11%。3个群体的群体内遗传相似度分别为0.7074±0.0469,0.7171±0.0475,0.7406±0.0449,香农多样性指数分别为0.185±0.128,0.194±0.119,0.176±0.138。变异来源有79.13%来自群体内,20.87%来自群体间。AFLP analysis of genetic diversity in the three populations of Chinese mitten crab Eriocheir sinensis from Liaohe River,Japanese mitten crab(E.japonica) from Russia and their hybrids was carried out.Total 233 loci were amplified with 4 primer pairs,and the percentage of polymorphic loci was 86.32%,86.75% and 85.9% in the three populations,respectively.The Shannon diversity index for Chinese mitten crab,Japanese mitten crab,and their hybrid populations was 0.185±0.128,0.194±0.119 and 0.176±0.138,respectively...

关 键 词：中华绒螯蟹 日本绒螯蟹 遗传多样性 AFLP分析 

分 类 号：S917.4[农业科学—水产科学]