犬肝移植模型肝动脉缺血对血清TGF-β1值的影响及其意义  

Influence and significance of hepatic artery ischemia on the value of serum TGF-β1 in liver transplantation model of dog

作　　者：陆宏伟[1] 黎一鸣[1] 吉鸿[1] 徐金锴[1] 

机构地区：[1]西安交通大学医学院第二附属医院普外科,西安710004

出　　处：《陕西医学杂志》2008年第2期138-140,共3页Shaanxi Medical Journal

摘　　要：目的:研究犬肝移植模型中,肝动脉缺血对血清中TGF-β1值的影响及其意义。方法:24只杂交犬被随机分为正常对照组、肝动脉门静脉同时开放组和肝动脉缺血组,每组8只。肝动脉门静脉同时开放组在制模后,同时开放肝动脉和门静脉,并在肝动脉和门静脉开放后0h、2h、6h、12h和24h分别抽血留样;肝动脉缺血组在制模后,先开放门静脉,2h后再开放肝动脉,在肝动脉开放后0h、2h、6h、12h和24h分别抽血留样;正常对照组开腹后仅游离肝脏并解剖相应的血管,在此之后0h、2h、6h、12h和24h分别抽血留样。ELISA法测定血清中的TGF-β1值。结果:正常对照组随着手术时间的延长,其血清中TGF-β1值无显著变化;肝动脉门静脉同时开放组和肝动脉缺血组在制模后,随着时间的延长,其血清中TGF-β1值逐渐升高,肝动脉门静脉同时开放组各时点之间无显著性差异(P>0.05),肝动脉缺血组各时点值之间有显著性差异(P<0.05);TGF-β1值在3组各时点相比较差异显著(P<0.05)。结论:在犬肝移植模型中,肝动脉缺血可引起血清中TGF-β1值显著升高。Objective:To study the influence and the significant of hepatic artery ischemia on the value of serum TGF-β1 in the liver transplantation model of dog.Methods:24 dogs were divided into normal control group,open hepatic artery and portal vein group(HPOS) and hepatic artery ischemia group(HAI) randomly,each group having 8 dogs.After the models were induced,the hepatic artery and portal vein were opened simultaneously and the blood samples were drawn at 0h,2h,6h,12h and 24h in the HPOS group;in the hepatic art...

关 键 词：肝移植 肝动脉缺血 转移生长因子Β1 犬 

分 类 号：R657.3[医药卫生—外科学]