五叶地锦离体培养及植株再生  

In vitro Culture and Plant Regeneration of Parthenocissus quinquefolia

作　　者：尹凌波[1] 陈颖[1] 孙振元[2] 杨学军[2] 赵梁军[1] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100094 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2008年第1期96-100,共5页Forest Research

基　　金：国家科技支撑计划:"优质抗逆专用地锦新品种选育"(2006BAD01A19-5)

摘　　要：以五叶地锦(Parthenocissus quinquefolia)子叶柄、下胚轴、叶片为外植体进行离体培养,以1/2 MS、B5和Heller为基本培养基,附加不同浓度的细胞分裂素(6-BA,KT,TDZ)及生长素(NAA)诱导下胚轴直接再生不定芽。实验结果表明,暗处理30 d,靠近下胚轴的子叶柄接种在1/2 MS+0.3 mg.L-16-BA培养基上,其不定芽分化率最高达64.67%;下胚轴接种在1/2 MS+2.0 mg.L-1KT+0.05 mg.L-1NAA和Heller+0.5 mg.L-1BA+0.1 mg.L-1NAA培养基上,其不定芽分化率接近,达到24%左右;无菌苗的叶片接种在B5+0.5 mg.L-1TDZ+0.05 mg.L-1NAA培养基上,其不定芽分化率达17.7%。芽增殖培养基为1/2 MS+0.5 mg.L-16-BA+0.1 mg.L-1NAA增殖系数为3~5,1/2 MS+0.5 mg.L-1IBA+500 mg.L-1活性炭适于再生幼苗的生根,生根苗经移栽全部成活。Adventitious shoots were successfully regenerated from cotyledon petioles,hypocotyl explants and leaf explants of in vitro cultures of Parthenocissus quinquefolia.They were cultured on half strength Murashine and Skoog(MS),B_5and Heller basal medium supplemented with various plant growth regulators.Auxins,α-naphthaleneacetic acid(NAA) and in combination with three cytokinins,benzylaminopurine(BA),kinetin(KT) and thidiazuron(TDZ) were tried.The study showed that the highest regeneration rate were obtained by...

关 键 词：五叶地锦 子叶柄 下胚轴 叶片 植株再生 

分 类 号：S687[农业科学—观赏园艺]