肺炎衣原体特异性单克隆抗体的研制及应用  

Production and Application of Chlamydia Pneumoniae-specific Monoclonal Antibody

作　　者：王卫群 钱利生[2] 史益军 李雪萍[2] 白永怿 徐坚 余竹元 

机构地区：[1]复旦大学附属金山医院心内科,上海200540 [2]复旦大学上海医学院病原生物学系微生物学教研室,上海200540

出　　处：《生物医学工程学杂志》2008年第3期658-661,共4页Journal of Biomedical Engineering

基　　金：上海市卫生局科研基金资助项目(沪卫科教[2002]58号2002-024006)

摘　　要：采用肺炎衣原体(Chlamydia pneumoniae,Cpn)TW-183的纯化原体免疫BALB/C雄性小鼠,将小鼠脾脏细胞与SP2/0细胞融合,杂交细胞通过有限稀释法克隆,获得1株稳定分泌Cpn-单克隆抗体(Cpn-McAb)的细胞株,单抗属IgG2b亚型及抗Cpn-MOMP抗体。自制Cpn-McAb与鹦鹉热衣原体原体有较弱的交叉反应;与沙眼衣原体原体无交叉反应,特性与进口Cpn-McAb一致。为了评估Cpn-McAb,用自制和进口Cpn-McAb同时检测外周血单核细胞标本454例,Cpn-Ag阳性率分别为53·3%和52·6%,Kappa值0·714,两者有较高的一致性。结果提示,自制Cpn-McAb几乎和进口Cpn-McAb一样有较高的特异性和敏感性,可替代进口单抗用于临床Cpn感染的检测,有助于相关疾病的诊治。The purified elementary bodies of C. pneumoniae TW-183 were used for immunization of male BALB/c mice, the spleen cells of these mice were fused with SP2/0 cells and the hybrid cells were cloned by limiting dilution. One clone that secreted the C. pneumoniae monoclonal antibody (Cpn-McAb) stably was obtained finally. The Cpn-McAb belonged to IgG2b class and anti-Cpn-MOMP; the outcome of micro-immunofluorescence showed its weak cross reaction with the C. psittaci elementary body but it has no cross reaction ...

关 键 词：肺炎衣原体特异性单克隆抗体 微量免疫荧光 外周血单核细胞 

分 类 号：R392[医药卫生—免疫学]