铜绿微囊藻生物钟蛋白KaiC的自激活活性和自身相互作用研究  

Studies on self-activation and self-interaction of circadian clock protein KaiC of Microcystis aeruginosa in yeast two hybridization

作　　者：王靖[1] 徐虹[1] 李珣[1] 郑锦乾[1] 王立红[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《海洋环境科学》2008年第1期67-70,82,共5页Marine Environmental Science

基　　金：国家自然科学基金

摘　　要：通过PCR扩增了铜绿微囊藻的生物钟主控基因KaiC,并将其分别克隆到酵母双杂交系统的诱饵质粒pGBKT7和猎物质粒pGADT7中,然后将重组质粒pGBKT7-kaiC/pGADT7-kaiC和pGBKT7-kaiC/pGADT7分别共转化酵母菌AH109,经营养缺陷生长和β-半乳糖苷酶印迹检测表明,KaiC蛋白不具有毒性不会影响酵母细胞的生长,也不具有自激活活性,不会激活报告基因的表达,并且KaiC蛋白自身存在较强的相互作用。诱饵质粒pGBKT7-kaiC可用于从基因组文库中筛选KaiC的相互作用蛋白。A clock system is universal among cyanobacteria.In this study,the clock gene kaiC was cloned from Microcystis aeruginosa by PCR.Through the study of the function and finding its interacting protein,the kaiC gene in Microcystis aeruginosa was subcloned into the bait vector pGBKT7 and prey vector pGADT7 of yeast two-hybrid system.After being verified by sequencing,the recombinant plamids pGBKT7-kaiC/pGADT7-kaiC and pGBKT7-kaiC/pGADT7 were co-transferred into yeast strain AH109 by polyethylene glycol/lithium a...

关 键 词：铜绿微囊藻 钟蛋白KaiC 酵母双杂交 

分 类 号：Q946[生物学—植物学]