阿霉素对心肌细胞损伤的实验研究  

Research on the Damage of Myocardial Cells induced by Adriamycin

作　　者：徐久宏[1] 张三典[2] 高耀明[1] 张军宁[1] 

机构地区：[1]苏州大学附属第一医院肿瘤放疗科,江苏苏州215006 [2]浙江李惠利医院肿瘤放疗科,浙江宁波315041

出　　处：《苏州大学学报（医学版）》2008年第1期47-49,共3页Suzhou University Journal of Medical Science

摘　　要：目的研究阿霉素对体外培养乳鼠心肌细胞的损伤作用。方法采用MTT法观察阿霉素对乳鼠心肌细胞活性的影响;通过检测乳酸脱氢酶(LDH)释放量,观察阿霉素作用下的乳鼠心肌细胞的坏死程度;采用流式细胞仪检测阿霉素诱发的心肌细胞凋亡百分率。结果(1)MTT实验发现:阿霉素浓度在0.3～10.0μg/ml均能抑制心肌细胞活性并成剂量依赖性;(2)细胞心肌酶谱检测到LDH释放量与阿霉素浓度呈正相关;(3)流式细胞仪发现心肌细胞凋亡率与阿霉素浓度有相关性。结论一定浓度的阿霉素对心肌细胞有毒性作用,可致心肌细胞损伤,表现为心肌细胞坏死和凋亡发生率增加。Objective To study the damage induced by adriamycin(ADM) in cultured murine myocardial cells.Methods After exposed to adriamycin,the viability of cardiac muscle cells was assessed by MTT colorimetric assay and apoptosis rate was determined by flow cytometry,the lactate dehydrogenase(LDH) in myocardial was examined by blood biochemical autoanalyzer.Results(1)Adriamycin inhibited the growth of rat cardiac muscle cells and had a dose-dependment or time-dependment manner in a certain range of dose.(2) The relat...

关 键 词：阿霉素 体外培养乳鼠心肌细胞 凋亡 

分 类 号：R96[医药卫生—药理学]