进境美国小麦中黑麦草腥黑穗病菌的检测和鉴定  

Detection and identification of Tilletia walkeri from imported US wheat

作　　者：田利华[1] 易建平[2] 周国梁[2] 周业琴[3] 印丽萍[2] 周而勋[1] 

机构地区：[1]华南农业大学,广州510642 [2]上海出入境检验检疫局 [3]江苏大学

出　　处：《植物检疫》2006年第S1期27-30,共4页Plant Quarantine

基　　金：上海市科委项目(04DZ05002);上海市科委重大攻关项目(03DZ19315)

摘　　要：从进境美国小麦洗涤液中发现一种类似小麦印度腥黑穗病菌的冬孢子,冬孢子大小为25.0~33.8(29.5±2.3)μm,球形至近球形,淡黄褐色至暗红褐色,半透明至不透明,外胞壁具深褐色疣状突起,突起表面观为锥形至平截状,有时呈不完全脑纹状,外被淡色透明胶质鞘。冬孢子处理后经通用引物Til1/Til4和U2/U10扩增,其扩增产物用黑麦草腥黑穗病菌Tilletiawalkeri的特异性引物W1/W4再次扩增可以得到760bp的产物,产物测序得到的序列和GenBank中黑麦草腥黑穗病菌菌株YRG-001(AF218063)的序列相似性为99%(717/719),和GenBank中小麦印度腥黑穗病菌菌株Bpop(AF218060)的序列相似性为96%(694/719)。实时荧光检测的结果表明黑麦草腥黑穗病菌的探针TWAL能得到阳性扩增曲线。根据冬孢子形态特征、PCR分析、实时荧光检测和序列分析的结果,将这种腥黑穗病菌冬孢子鉴定为黑麦草腥黑穗病菌(T.walkeri)。Tilletia indica-like teliospores are found in wheat(Triticum aestivum) samples that imported from New Orleans USA to Shanghai in August 2006. The teliospore is globose to subglobose,25.0~33.8(29.5±2.3μm diam, pale yellow to dark reddish-brown, opaque to subopaque. Tuberculate teliospore has exospore ornamentation with conical to truncate projections which from surface view appear coarsely and incompletely cerebriform to coralloid; with hyaline to yellowish sheath extending to tips of projections. A PCR prod...

关 键 词：小麦 Tilletia walkeri 检测 鉴定 

分 类 号：S41-30[农业科学—植物保护]